阿伐他汀对辐射致血管内皮细胞损伤的保护作用研究

目的 观察阿伐他汀对血管内皮细胞(VEC)血栓调节素(TM)表达的影响和辐射损伤的防护作用。方法 体外培养人脐静脉内皮细胞和主动脉内皮细胞,用阿伐他汀10 μmol／ml作用于生长期内皮细胞1 h,分别经γ射线照射2和25 Gy,24 h后检测VEC 的TM表达、蛋白C含量及细胞形态学变化。结果 阿伐他汀处理后24 h,正常对照组VEC表面TM表达水平迅速提高了77%(t=27.395,P＝0.000),2和25 Gy照射组分别提高59%(t=26.420,P=0.000)和61%(t=58.065,P=0.000)。2和25 Gy照射后,蛋白C含量比正常VEC组下降23%和34%,但阿伐他汀处理后分别比各自对照组增加79%和76%。照后24h,阿伐他汀处理可使2和25 Gy照射组VEC凋亡率分别降低6%和16%(t=4.178,P=0.006;t=17.863,P=0.000)。结论 阿伐他汀可显著增强VEC的TM表达,激活蛋白C的形成,从而增强内皮细胞抗凝血与抗炎功能,并有效减轻辐射所致的内皮细胞损伤。

Protective effect of atorvastatin on radiation-induced endothelial cell injury

Objective To explore the protective effect of atorvastatin on irradiated endothelium and the thrombomodulin(TM)expression.Methods Cultured human coronary artery endothelial cells(HCAEC)and human umbilical vein endothelial cells(HUVEC)were treated by atorvastatin at the final concentration of 10 μmol/ml for 10 min,and then irradiated with 2 and 25 Gy.Cell cycles status and TM expression were quantitatively measured by flow cytometry 24 hours after irradiation.Protein C activation in endothelial cells was also assessod.Results After administration with atorvastatin for 24 h,the TM expression increased by 77%,59% and 61% in normal control group,2 Gy group and 25 Gy group,respectively(t=27.395,26.420,58.065;P=0.000).The protein C levels decreased by 23% and 34% compared with the normal group post-irradiation to 2 and 25 Gy,but increased by 79% and 76% compared with the irradiated control group after administration with atorvastatin.The rates of cell apoptosis decreased by 6% and 16% in 2 Gy and 25 Gy groups,respectively after administration with atorvastatin for 24 h(t=4.178,17.863;P=0.000).Conclusions Atorva statin can protect endothelia cell from irradiation-induced apeptosis by increasing TM expression and protein C activation.