BUB1在喉鳞状细胞癌中的表达及临床意义

目的：探讨喉鳞状细胞癌（LSCC）组织中BUB1蛋白的表达及临床意义。方法：采用免疫组织化学EliVision二步法检测55例LSCC组织和30例癌旁正常黏膜组织中BUB1蛋白的表达，并分析其与LSCC临床病理特征的关系。结果：BUB1蛋白在LSCC组织中的阳性表达率为50．9％（28／55），低于癌旁正常黏膜组织83．3%（25／30），两者比较差异有统计学意义（P〈0．01）；BUBl蛋白的表达与LSCC分化程度及淋巴结转移有相关性（均P〈0．05），而与患者性别、年龄、吸烟、肿瘤原发部位、T分期及临床分期无相关性（均P〉0．05）。结论：BUBl蛋白的低表达与LSCC的发生、发展有关，BUB1可能作为预测LSCC转移及预后的生物学指标之一。

The expression of BUB1 carcinoma and its in laryngeal squamous cell clinical significance

Objective：To investigate the expression and clinical significance of BUB1 protein in laryngeal squamous cell carcinoma（LSCC） tissues. Method：The expression of BUB1 protein was measured by immunohistochemistry（EliVision two steps） in 55 cases of LSCC tissues,30 cases of adjacent normal laryngeal mucosa tissues, the relationship of BUB1 expression with clinical pathologic factors in LSCC was also analyzed. Result： The positive rate of BUB1 protein in LSCC tissues（50.9 %, 28/55） was significantly lower than that in adjacent normal laryngeal mucosa tissues（83.3 %, 25/30）, P〈0.01. The positive rate of BUB1 protein was correlated with differentiation grading and lymph node metastasis of LSCC（P〈0.05, respectively）, but not with patient＇s sex, age, smoking, tumor site, T-stage and clinical stage（P〈0.05,respectively）. Conclusion：The low expresssion of BUB1 protein is closely related to the tumorigenesis and development of LSCC, and can be a molecular marker for prognosticating metastasis and prognosis of LSCC.