BRCA1基因启动子区甲基化状态抑癌作用研究

目的：研究BRCA1基因启动子区甲基化状态在乳腺癌组织中的抑癌作用。方法甲基化PCR法检测60例乳腺癌患者的癌组织、癌旁组织和20例乳腺良性组织中BRCA1-mRNA表达及启动子区甲基化状态，以及评价其作用。结果癌组织和癌旁组织中BRCA1-mRNA的表达水平分别为（0.6122＆#177;0.2344）、（0.6875＆#177;0.2576），在癌组织、癌旁组织、良性肿瘤组织中BRCA1表达的甲基化率分别为70.0%、48.3%、35.0%。癌组织的甲基化率明显的高于癌旁组织和良性肿瘤组织；经统计学分析显示，淋巴结转移、肿瘤分级在BRCA1-mRNA表达及启动子区甲基化中的差异均具有统计学意义（P＜0.05），绝经、组织分型在BRCA1-mRNA表达及启动子区甲基化中的差异均无统计学意义（P＞0.05）。结论 BRCA1-mRNA表达及启动子区甲基化状态在乳腺癌的发生发展中起着重要的作用，并且与组织分期和淋巴结的转移密切相关。

Effect of BRCA1 promoter methylation on tumor inhibition

Objective To study the effect of BRCA1 promoter methylation on tumor inhibition in breast can-cer tissue. Methods BRCA1-mRNA expression and promoter methylation were detected by methylation PCR in 60 cases of breast cancer tissue, pericarcinous tissue, and 20 cases of benign mammary gland tissue. Meanwhile, its ef-fect was evaluated. Results The expression of BRCA1-mRNA levels in the cancer tissue and pericarcinous tissue were （0.612 2＆#177;0.234 4） and （0.234 4 ＆#177;0.257 6）, respectively. The methylation rate in cancer tissure, pericarcinous tis-sue and benign tumor tissue were 70.0%, 48.3%and 35.0%, respectively. And the methylation rate in cancer tissue was significantly higher than those in pericarcinous tissue and benign tumor tissue. The differences of lymph node ma-tastasis and tumor classification in BRCA1 mRNA expression and promoter methylation were statistically significant （P〈0.05）, while there was no difference in menopause and histological stages in BRCA1 mRNA expression and pro-moter region methylation （P〉0.05）. Conclusion BRCA1 mRNA expression and promoter region methylation status play an important role in the development of breast cancer, and are closely related to the histological stages and lymph node metastasis.