| RP-HPLC法测定牡丹皮提取物中丹皮酚的含量 |
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| 引用本文： | 刘经亮,刘纲勇,王如意,黄海潮.RP-HPLC法测定牡丹皮提取物中丹皮酚的含量[J].海峡药学,2014,26(1):81-82. |
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| 作者姓名： | 刘经亮 刘纲勇 王如意 黄海潮 |
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| 作者单位： | 刘经亮 (广东食品药品职业学院,广州,510520)； 刘纲勇 (广东食品药品职业学院,广州,510520)； 王如意 (广东食品药品职业学院,广州,510520)； 黄海潮 (广东食品药品职业学院,广州,510520)； |
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| 基金项目： | 广东省中医药局基金项目(项目编号：20111279) |
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| 摘 要： | 目的 建立RP-HPLC测定牡丹皮提取物中丹皮酚含量的方法.方法 采用RP-HPLC方法测定丹皮酚的含量.固定相为 Inertsil ODS-3 C18反相柱（4.6mm×250mm,5μm）,以甲醇-水（60：40）为流动相,流速0.8mL·min-1,检测波长274nm,柱温30℃,进样量10μL.结果 丹皮酚的线性范围为35.8～286.4μg·mL-1（r=0.9999）,平均回收率为98.54%,RSD=1.03%,（n=6）.提取方法为超声辅助水蒸汽蒸馏.结论 本方法简便,灵敏,准确,重复性好,可为牡丹皮中防晒活性成分丹皮酚的含量检测提供依据.
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| 关 键 词： | 牡丹皮 丹皮酚 反相高效液相色谱法 含量测定 |
Determination of paeonol in extract of Cortex Moutan by RP-HPLC |
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| LIU Jing-liang,LIU Gang-yong,WANG Ru-yi,HUANG Hai-chao.Determination of paeonol in extract of Cortex Moutan by RP-HPLC[J].Strait Pharmaceutical Journal,2014,26(1):81-82. |
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| Authors: | LIU Jing-liang LIU Gang-yong WANG Ru-yi HUANG Hai-chao |
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| Institution: | (Guangdong Food and Drug Vocational College, Guangzhou .5 ]0520, China) |
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| Abstract: | OBJECTIVE To develop a RP-HPLC method for the determination and analysis of paeonol in ex- tract of cortex moutan. METHODS The content of Paeonol was determined by RP-HPLC method. The analysis was performed on a RP-HPLC apparatus equipped with a Inertsil ODS-3 C18 column （4. 6mm × 250mm,5μm） ,The DAD detection was carried out at the wavelength of 274nm and a temperature of 30℃, the mobile phase consisted of meth- anol-water （60：40） with the flow rate of 0. 8mL · rain-1. RESULTS The calibration curve of paeonol was linear in the ranges of 35.8 μg· mL- 1 （ R2 = 0. 9999 ）. The average recovery of paeonol was 98.54% （ RSD = 1.03%, n = 6） . The sample was extracted with water-steam distillation after ultrasonication 30min. CONCLUSION The method is effective, accurate, and can be applied to quantitatively analyse paeonol in extract of cortex moutan. |
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| Keywords: | Cortex moutan Paeonol RP-HPLC Content determination |
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