运用组织芯片检测淋巴瘤中活化的细胞毒性细胞的意义

目的运用组织芯片及常规技术检测活化的细胞毒性细胞在各型淋巴瘤中的表达分布情况,为临床治疗和判断预后提供依据.方法运用免疫组织化学方法检测以60例淋巴瘤标本制成的淋巴瘤组织芯片中穿孔素、颗粒酶B的表达和分布情况;同时选择10例鼻NK/T细胞淋巴瘤常规标本与组织芯片进行比较研究,10例淋巴组织反应性增生作为对照.结果在60例淋巴瘤制成的组织芯片中,发生在淋巴结内者48例,淋巴结外者12例.B细胞淋巴瘤42例,T细胞淋巴瘤16例,2例霍奇金淋巴瘤.42例B细胞淋巴瘤的瘤细胞均不表达穿孔素和颗粒酶B.10例外周T细胞淋巴瘤中8例表达穿孔素和9例表达颗粒酶B,阳性表达细胞均为非肿瘤细胞.芯片中的2例和常规10例鼻NK/T细胞淋巴瘤中均见穿孔素和颗粒酶B过度表达.T、B细胞淋巴瘤与NK/T细胞淋巴瘤比较有显著性差异（P〈0.01）.结论穿孔素和颗粒酶B是鉴定活化的细胞毒性细胞的免疫标志物,可作为NK/T细胞淋巴瘤的诊断性标志物;其在T细胞淋巴瘤中的表达反映了机体存在抗肿瘤的免疫反应机制.组织芯片技术具有高信息量、简捷、高效、实验误差小、重复性好等优点,可作为研究淋巴瘤的有用工具.

Detection of activated cytotoxic cells in lymphoma by tissue microarray

Objective To detect the expression and distribution of activated cytotoxic cells in types of lymphoma with tissue microarray, and provide evidences for clinical treatment and prognosis. Methods Immunohistochemical staining by S-P technique was used to detect the expression and distribution of perforin and granzyme B in the lymphoma tissue microarray, composed of 60 cases of lymphoma tissue. Ten cases of NK/T-cell lymphoma routine sections were used for relative research, and ten cases of reactive hyperplasia were used for comparison. Results In the tissue microarray, originated from intranode and extranode were 48 cases and 12 cases, respectively; consisting of 42 cases of B-cell lymphoma, 16 cases of T-cell lymphoma, two cases of Hodgkin's disease. 42 cases of B-cell lymphoma cells were negative in perforin and granzyme B. In Ten cases of peripheral T-cell lymphoma, perforin and granzyme B positive were eight cases and nine cases, respectively, but the positive cells were no tumor cells. In 12 cases of NK/T-cell lymphoma (two in the tissue microarray, ten routine sections), both perforin and granzyme B were strongly positive. B-cell lymphoma, T-cell lymphoma and NK/T-cell lymphoma differed significantly (P<0.01). Conclusion Perforin and granzyme B were immunity markers for the identification of activated cytotoxic cells, which also could be used as diagnostic markers of NK/T-cell lymphoma. Their expression in B-cell lymphoma reflected the anti-tumor immunologic reaction of host. Tissue microarray technology has the behavior of high-throughput, convenient, effective, small experiment error, good replication and so on, and can be used as a useful tool for research of lymphoma.