| 人血管内皮细胞在白血病小鼠中参与形成微血管的研究 |
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| 引用本文： | 郭海霞,梁立阳,吴燕云,陈纯.人血管内皮细胞在白血病小鼠中参与形成微血管的研究[J].中山大学学报(医学科学版),2008,29(1):42-45. |
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| 作者姓名： | 郭海霞 梁立阳 吴燕云 陈纯 |
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| 作者单位： | 中山大学附属第二医院儿科,广东,广州,510120 |
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| 摘 要： | 【目的】研究人血管内皮细胞在建立NOD/SCID小鼠白血病微血管中的作用。【方法】NOD/SCID小鼠分3组:A空白对照组,B注射EC组,C建立白血病模型后注射EC组。通过免疫组化对NOD/SCID小鼠骨髓的血管生成及全身肿瘤负荷情况进行评价。【结果】C组骨髓微血管密度明显大于A、B组,白血病新生的微血管分支多紊乱,管腔较不规则,血管形态幼稚。【结论】用人血管内皮细胞成功建立NOD/SCID小鼠白血病微血管生成模型,可用于抗白血病血管生成及相关实验的研究。
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| 关 键 词： | 血管生成 人血管内皮细胞 白血病 |
| 文章编号： | 1672-3554(2008)01-0042-04 |
| 收稿时间： | 2007-03-25; |
| 修稿时间： | 2007年3月25日 |
Study of Human Endothelial Cell on Construction of Microvessel Angiogenesis in Leukemia Mice |
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| GUO Hai-xia,LIANG Li-yang,WU Yan-yun,CHEN Chun.Study of Human Endothelial Cell on Construction of Microvessel Angiogenesis in Leukemia Mice[J].Journal of Sun Yatsen University(Medical Sciences),2008,29(1):42-45. |
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| Authors: | GUO Hai-xia LIANG Li-yang WU Yan-yun CHEN Chun |
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| Abstract: | Objective] To study the role of human vascular endothelial cell in angiogenesis in NOD/SCID leukemia mice. Methods] NOD/SCID mice were divided into 3 groups: A, blank group; B, EC injection group; C, leukemia model mice with EC injection group. MVD in bone marrow with cnemis slice were calculated after immunohistochemistry dyeing. Leukemia burden was evaluated in leukemia model mice. Results] Bone marrow MVD in group C was significantly higher than that in group B. Microvessels in leukemia mice had disordered branches and irregular cavity. The morphism of vessels was immature. Conclusion] Microvessel angiogenesis model in NOD/SCID leukemia mice has been constructed successfully with human endothelial cell. It can be used in the study of anti-angiogenesis in leukemia and relative researches. |
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| Keywords: | angiogenesis human vascular endothelial cell leukemia |
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