10-23型脱氧核酶对HBV S、C基因体外切割作用研究 |
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引用本文: | 江京娣,张晓洁,张爱霞,位春芳,徐茹,葛建顺,钱中清,吕静竹.10-23型脱氧核酶对HBV S、C基因体外切割作用研究[J].蚌埠医学院学报,2017,42(12):1573-1575. |
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作者姓名: | 江京娣 张晓洁 张爱霞 位春芳 徐茹 葛建顺 钱中清 吕静竹 |
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作者单位: | 蚌埠医学院生物化学与分子生物学教研室,安徽 蚌埠233030;蚌埠医学院免疫学教研室,安徽 蚌埠233030;蚌埠医学院感染与免疫安徽省重点实验室,安徽 蚌埠,233030;蚌埠医学院临床医学院,安徽 蚌埠,233030 |
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基金项目: | 国家自然科学基金项目,安徽省重点研究,开发计划(对外科技合作项目),安徽省高等学校省级自然科学研究重点项目,国家级大学生创新创业训练计划项目 |
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摘 要: | 目的:探讨10-23脱氧核酶(10-23 DRz)对HBV S基因及C基因体外转录产物的特异性切割作用.方法:分别构建含有HBV S基因或C基因的重组质粒,以线性化的重组质粒为模板,体外转录获得相应HBV S基因RNA及C基因RNA.设计并合成针对HBV S基因的DRz-hbvs-1和针对C基因的DRz-hbvc-1,观察其对靶RNA的体外切割作用.结果:DRz-hbvs-1及DRz-hbvc-1能对各自相应的靶RNA进行有效的特异性切割;无DRz对照组及反义寡核苷酸对照组均未见特异性切割.结论:10-23 DRz能特异性切割HBV S基因及C基因体外转录产物.
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关 键 词: | 乙型肝炎病毒 脱氧核酶 基因治疗 |
Cleavage effect of type 10-23 deoxyribozyme on HBV S and C gene in vitro |
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Abstract: | Objective:To explore the specific cleavage activities of type 10-23 deoxyribozyme 10-23 DRz enzymes on HBV S and C RNA in vitro. Methods:The recombinant plasmids containing HBV S and C gene were constructed,respectively. The HBV S or C gene RNA was synthesized in vitro using the linearized recombinant plasmid as template. The DRz-hbvs-1 and DRz-hbvc-1 targeting to cleave HBV S and C mRNA were designed and synthesized,respectively,and the cleavage activities of which on targeting RNA were observed in vitro. Results:The DRz-hbvs-1 and DRz-hbvc-1 could specifically and effectively cleave the targeting RNA,and the cleavage of which in nonDRz and ASODN control groups were not found. Conclusions:The 10-23 DRz can specifically cleave the HBV S and C gene RNA in vitro. |
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Keywords: | hepatitis B virus deoxyribozyme gene therapy |
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