Villin和VEGF在结直肠腺癌组织中的表达及临床病理意义

目的 探讨绒毛蛋白(Villin)和血管内皮生长因子(VEGF)在结直肠腺癌组织中的表达及临床病理意义.方法 采用免疫组化方法检测40例结直肠腺癌、10例结直肠腺癌切缘黏膜、10例结直肠腺瘤(轻-中度不典型增生5例,重度不典型增生5例)组织中Villin和VEGF的表达情况,并对二者表达的相关性及与各临床病理特征的关系进行统计学分析.结果 Villin在结直肠腺癌切缘黏膜、结直肠腺瘤(轻-中度不典型增生、重度不典型增生)及结直肠腺癌中的阳性表达率分别为90.0％、70.0％(80.0％、60.0％)及50.0％,呈递减趋势,3组比较,差异有统计学意义(P=0.029);切缘黏膜的Villin阳性表达率高于结直肠腺癌,差异有统计学意义(P﹤0.05);Villin的表达与结直肠腺癌的病理分级、淋巴结是否发生转移以及临床病理分期相关(P﹤0.05).VEGF在切缘黏膜、结直肠腺瘤(轻-中度不典型增生、重度不典型增生)及结直肠腺癌中的阳性表达率分别为30.0％、50.0％(40.0％、60.0％)及72.5％,呈递增趋势,3组比较,差异有统计学意义(P=0.024);切缘黏膜的VEGF阳性表达率低于结直肠腺癌,差异有统计学意义(P﹤0.05);VEGF的表达与结直肠腺癌的病理分级及肌层浸润深度相关(P﹤0.05).结直肠腺癌组织中Villin和VEGF的表达不存在相关性(P﹥0.05).结论 Villin和VEGF在结直肠黏膜恶性转变中发挥一定作用,可能参与结直肠腺癌的浸润和转移.在结直肠腺癌中同时检测Villin和VEGF的表达情况,可能对判断结直肠腺癌的发生、发展及恶变程度具有一定价值,并可能有助于预测其生物学行为.

Expression and clinical pathological significance of Villin and VEGF in colorectal adenocarcinoma

Objective To investigate the expression and clinical pathological significance of Villin and VEGF in colorectal adenocarcinoma. Method The expression of Villin and VEGF were detected by immunohistochemistry in 40 cases of colorectal adenocarcinoma, 10 cases of resected colorectal mucosa of colorectal adenocarcinoma, 10 cases of colorectal adenomas (5 cases with mild to moderate dysplasia and 5 with severe dysplasia). Then, the relationships be-tween the expression levels and their clinicopathological features were studied by statistical analysis. Result The posi-tive rates of expression of Villin in resected colorectal mucosa, colorectal adenoma (mild to moderate and severe dyspla-sia) and colorectal adenocarcinoma were 90.0％, 70.0％(80.0％and 60.0％) and 50.0％, respectively, with a decreasing trend observed, and the differences among these groups were significant (P=0.029);resected colorectal mucosa had signif-icantly higher positive rate compared with that in colorectal adenocarcinoma (P<0.05);the expression of Villin in colorec-tal adenocarcinoma was correlated with the pathological grading, lymphatic metastasis and clinical staging (P<0.05). The positive expression rate of VEGF in resected colorectal mucosa, colorectal adenoma and colorectal adenocarcinoma (mild to moderate and severe dysplasia) were 30.0％, 50.0％(40.0％and 60.0％) and 72.5％, respectively, instead, an increasing trend was observed, and the differences were significant (P=0.024);VEGF expression was associated with the pathologi-cal grading and depths of myometrial invasion (P<0.05). Villin expression was independent of VEGF expression in colorectal adenocarcinoma (P>0.05). Conclusion Villin and VEGF are involved in the malignant transformation of colorectal mucosa, and probably the infiltration and metastasis of colorectal adenocarcinoma. Simultaneous detection of Villin and VEGF expressions in colorectal adenocarcinoma may be helpful in the judgment of the development, progres-sion and malignant degree and aid to predict the biological behaviors of colorectal adenocarcinomas.