急性肝衰竭大鼠肝组织SOCS表达及意义

目的研究急性肝功能衰竭(acute liver failur,ALF)大鼠肝组织细胞因子信号转导抑制因子(SOCSs)基因表达的动态变化及意义。方法腹腔注射D-胺基半乳糖(D-GalN)、脂多糖(LPS)建立急性肝衰竭大鼠模型,D-GalN和LPS剂量分别为800 mg/kg和8μg/只,分别在注射D-GalN、LPS后2,6,12,24,48 h 5个时间点留取大鼠血及肝脏标本。观察大鼠肝功能及肝脏病理变化;逆转录聚合酶链反应法(RT-PCR)检测动物肝组织中SOCS-1和SOCS-3mRNA表达;ELISA法测定血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平。结果肝衰竭大鼠肝组织SOCS-1和SOCS-3mRNA表达均明显上调,在造模后2 h即明显高于对照组,分别于6,12 h达高峰值。血清TNF-α、IL-6产生均显著增多(P<0.01),均于造模后6 h达峰值。结论急性肝功能衰竭可诱导体内SOCSs表达上调,其改变与TNF-α、IL-6水平的改变密切相关,提示其可能参与急性肝功能衰竭时炎症反应平衡的调节过程。

Dynamic changes of cytokine signaling suppressor mRNA in rats with acute liver failure

ObjectiveTo observe the expression of cytokine signaling suppressors(SOCSs)mRNA in rats with acute liver failure(ALF).MethodsThe model of ALF was induced in rats by D-galactosamine/lipopolysaccharide(D-GalN/LPS).Then,the gene expressions of SOCS-1 and SOCS-3 in the liver was determined by RT-PCR,the TNF-α and IL-6 level was estimated by ELISA at 2 h,6 h,12 h,24 h and 48 h after the injection of D-GalN/LPS.ResultsThe TNF-α and IL-6 level were significantly elevated and reached the peak at 6th hour(P<0.01).Meanwhile,the gene expressions of SOCS-1 and SOCS-3 were obviously higher than those in the controls and reached the peak at 6th and 12th hour respectively after the injection of D-GalN/L PS(P<0.01).ConclusionAcute liver failure can induce the up-regulation of the gene expressions of SOCSs in rats,which closely correlates to the changes of TNF-α and IL-6,indicating that SOCSs may participate in regulating the balance of inflammatory response in acute liver failure.