Lactate Dehydrogenase Isoenzyme Pattern: Marker for Differentiation of Lymphocytes |
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| Authors: | J. PLUM R. MONTEYNE E. DHONT M. DE SMEDT |
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| Affiliation: | Department of Medical Microbiology, University Hospital, Ghent, Belgium;Department of Radiotherapy and Nuclear Medicine, University Hospital, Ghent, Belgium |
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| Abstract: | The lactate dehydrogenase isoenzyme pattern has been determined in different murine thymocyte cell populations. Enrichment of thymocytes with more mature cells through total irradiation results in a smaller percentage of LDH-1, LDH-2, LDH-3 and a greater percentage of LDH-4 and LDH-5. Fractionation of normal thymocytes by velocity sedimentation at unit gravity yields a fraction of cells with a high sedimentation rate. LDH-1 and LDH-2 formed a smaller percentage of the total enzyme activity in these cells. These findings indicate that the LDH isoenzyme distribution is a marker for differentiation of thymocytes. |
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