贵州苗族药酢浆草不同溶剂提取物的药效比较及HPLC测定

目的:研究酢浆草70%乙醇和水提取物的急性毒性、抗炎和镇痛作用,分析HPLC图中峰信息量。方法:采用急性毒性最大耐受量试验评价酢浆草70%乙醇和水提取物的急性毒性;冰乙酸致腹腔毛细血管通透性试验考察抗炎作用,冰醋酸致扭体反应考察镇痛作用;使用HPLC对不同溶剂提取物进行分析。结果:在35~1 120 mg·kg-1的剂量范围内各组小鼠活动正常,未见死亡,MTD大于1 120 mg·kg-1;与水提取物比较,70%乙醇提取物能更好地抑制冰乙酸引起的毛细血管通透性增高及扭体反应,HPLC峰信息更丰富,共有峰的提取率更高。结论:酢浆草70%乙醇和水提取物的致死剂量较高,毒性较小,70%乙醇提取物的抗炎镇痛作用优于水提取物,并且能够更充分的富集有效成分。

Comparison of Effects and HPLC Detection on Different Extracts of Miao Medicine Oxalis corniculata in Guizhou Province

Objective: To study acute toxicity, anti-inflammatory and anti-nociceptive effects of 70% ethanol extract (E_70%EtOH) and water extract (E_water) of Oxalis corniculata, HPLC was used to analyze figure information and determine the best extrat. Method: The maximum dosage test was used to evaluate safety of E_70%EtOH and E_water. Permeability increasion of celiac capillary induced by glacial acetic acid in mice was performed to investigate the anti-inflammatory effect, writhing test induced by glacial acetic acid in mice was used to observe the anti-nociceptive activity. Analyze different extracts by HPLC. Result: E_70%EtOHand E_water didn't show toxicity in the range of 35 to 1 120 mg · kg^-1, MTD>1 120 mg · kg^-1. Compare with E_water, E_70%EtOH could remarkable inhibit the permeability increasion of celiac capillary and the writhing reaction in mice, HPLC peak information is richer and the common peak ratio is higher than that of E_water. Conclusion: Lethal dose of E_70%EtOH and E_water may be very high and toxicity of E_70%EtOH and E_water may be very low. The effects of E_70%EtOH is better than that of E_water, E_70%EtOH can more fully the enrichment of active component.