山楂叶总黄酮对脑缺血再灌注损伤大鼠神经元凋亡和c-fos, c-jun表达的影响

目的:观察山楂叶总黄酮(TFHL)对脑缺血再灌注损伤大鼠神经元凋亡及c-fos,c-jun基因表达的影响。方法:80只SD大鼠随机分为假手术组、模型组、TFHL 70,140 mg·kg-1组和银杏叶片3.5 mg·kg-1组,每组16只。四血管阻断法建立全脑缺血再灌注损伤模型,穿梭箱实验检测学习记忆能力;分光光度比色法检测血清超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;酶联免疫吸附测定(ELISA)法检测半胱氨酸蛋白酶-3(caspase-3)的含量;免疫组化法检测c-fos及c-jun的表达。结果:与假手术组比较,模型组大鼠学习记忆能力显著降低,大鼠主动逃避反应率明显降低(P0.01),脑组织凋亡细胞显著增多(P0.01);c-fos与c-jun基因表达明显增强(P0.01);与模型组比较,TFHL及银杏叶片组大鼠主动逃避反应率增高(P0.05),SOD活性明显升高(P0.05),MDA含量显著降低(P0.05),凋亡细胞明显减少(P0.05),c-fos与c-jun基因表达显著降低(P0.05)。结论:TFHL可通过降低脑缺血再灌注损伤脑组织c-fos与c-jun基因表达、抑制神经元凋亡、改善脑缺血再灌注损伤大鼠的神经功能,提高大鼠的学习记忆能力。

Impact of Total Flavone of Hawthorn Leaf on Neuronal Apoptosis and c-fos and c-jun Expression in Rat Cerebral Ischemia-reperfusion Injury

Objective: To observe the total flavone of hawthorn leaf (TFHL) on apoptosis of cerebral ischemia reperfusion injury in rats and c-fos, c-jun the effects of gene expression. Method: Eighty SD rats were randomly divided into sham group, model group, TFHL 70 mg · kg^-1 group, 140 mg · kg^-1group and Yinxingye tablet 3.5 mg · kg^-1 group, and each group of 16. Four blood vessel blocking method to establish the whole cerebral ischemia reperfusion injury model of shuttle box test to detect the ability of learning and memory;spectrophotometric assay superoxide dismutase (SOD)activity and malondialdehyde(MDA) content;enzyme-linked immunosorbent assay (ELISA) method to detect the content of caspase-3;immunohistochemical method to detect c-jun and the expression of c-fos. Result: Compared with sham group, model group rats learning and memory ability, active rats escape response rate significantly decreased (P<0.01), Brain tissue apoptosis cells increased significantly (P<0.01);c-fos and c-jun gene expression obviously increased (P<0.01). Compared with model group, TFHL active escape response rate and Yinxingye tablet group rats increased (P<0.05), SOD activity was significantly increased (P<0.05), MDA content was significantly lower(P<0.05),apoptotic cells decreased significantly (P<0.05),c-fos and c-jun gene expression decreased significantly (P<0.05). Conclusion: TFHL can reducing cerebral ischemia reperfusion injury of brain tissue c-fos and c-jun gene expression, inhibition of neural cell apoptosis, improving cerebral ischemia-reperfusion injury in rats of nerve function, improve the learning and memory ability of rats.