| Apoptosis of human gastric adenocarcinoma cells induced by β-ionone |
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| 作者姓名： | Liu JR Chen BQ Yang BF Dong HW Sun CH Wang Q Song G Song YQ |
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| 摘 要： | AIM:To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901.METHODS: Using M-IT, fluorescence dye (Hoechst-33258),transmission electron microscopy and the TUNEL assay,we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations (i.e. 25, 50, 100 and 200μmol/L) for 24h,48h.RESULTS:The growth of SGC-7901 cells was inhibited by β-ionone. Seven days after treatment with β-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%,78.25% and 94.15%, respectively. The IC50 value of β-ionone for SGC-7901 cells was estimated to be 89μmol/L.The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in β-iononetreated SGC-7901 cells by the TUNEL assay.CONCLUSION:β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells.However, the mechanism needs to be further investigated.
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| 关 键 词： | β-紫罗酮 胃腺癌 肿瘤细胞 细胞凋亡 TUNEL法 发射电子显微镜 肿瘤抑制 |
| 收稿时间： | 2003 Aug 26 |
Apoptosis of human gastric adenocarcinoma cells induced by beta-ionone |
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| Liu JR,Chen BQ,Yang BF,Dong HW,Sun CH,Wang Q,Song G,Song YQ.Apoptosis of human gastric adenocarcinoma cells induced by beta-ionone[J].World Journal of Gastroenterology,2004,10(3):348-351. |
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| Authors: | Liu Jia-Ren Chen Bing-Qing Yang Bao-Feng Dong Hong-Wei Sun Chang-Hao Wang Qi Song Guo Song You-Qiang |
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| Institution: | Public Health College, Harbin Medical University, Harbin 15001, Heilongjiang Province, China. jiarl@ems.hrbmu.edu.cn |
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| Abstract: | AIM: To investigate the effect of beta-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901. METHODS: Using MTT, fluorescence dye (Hoechst-33258), transmission electron microscopy and the TUNEL assay, we examined growth and apoptosis of SGC-7901 cells treated with beta-ionone at various concentrations (i.e. 25, 50, 100 and 200 micromol/L) for 24 h, 48 h. RESULTS: The growth of SGC-7901 cells was inhibited by beta-ionone. Seven days after treatment with beta-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%, 78.25% and 94.15%, respectively. The IC(50) value of beta-ionone for SGC-7901 cells was estimated to be 89 micromol/L. The apoptotic morphology was demonstrated in SGC-7901 cells treated with beta-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in beta-ionone-treated SGC-7901 cells by the TUNEL assay. CONCLUSION: beta-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells. However, the mechanism needs to be further investigated. |
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