| DEVELOPMENT OF A VERSATILE SENSING MEMBRANE FOR IMMUBILIZATION OF PROTEIN AND A NEW IMMOBILIZATION METHOD |
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| 作者姓名： | Y.K. Zhou J. W. Yuan S. Q. Xu B. H. Shu |
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| 作者单位： | Y.K. Zhou，J. W. Yuan，S. Q. Xu，B. H. Shu(Department of Environmental Medicine，Tongji Medical University，Wuhan 430030，China |
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| 摘 要： | ＤＥＶＥＬＯＰＭＥＮＴＯＦＡＶＥＲＳＡＴＩＬＥＳＥＮＳＩＮＧＭＥＭＢＲＡＮＥＦＯＲＩＭＭＵＢＩＬＩＺＡＴＩＯＮＯＦＰＲＯＴＥＩＮＡＮＤＡＮＥＷＩＭＭＯＢＩＬＩＺＡＴＩＯＮＭＥＴＨＯＤＹ．Ｋ．Ｚｈｏｕ，Ｊ．Ｗ．Ｙｕａｎ，Ｓ．Ｑ．Ｘｕ，Ｂ．Ｈ．Ｓｈｕ（Ｄ...
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DEVELOPMENT OF A VERSATILESENSING MEMBRANE FORIMMUBILIZATION OF PROTEIN ANDA NEW IMMOBILIZATION METHOD |
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| Y.K. Zhou,J. W. Yuan,S. Q. Xu,B. H. Shu.DEVELOPMENT OF A VERSATILE SENSING MEMBRANE FOR IMMUBILIZATION OF PROTEIN AND A NEW IMMOBILIZATION METHOD[J].Chinese Journal of Biomedical Engineering,1996(4). |
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| Authors: | YK Zhou J W Yuan S Q Xu B H Shu |
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| Abstract: | This paper describes a versatile sensing membrane for immobilizationof proteins (antibodies, antigens, enzymes and so on) and a new immobolizationmethod' The membrane was developed with polyacrylaminde gel and blotinstrep-tavidin system. Streptavidin was bond to the polyacryamide gel with 1-ethyl-3-(3-dimethylaminopropyl carbodimide (EDAC), which results in a streptavidin membrane, and a biotinalyted ligand was then easily linked to the gel via the streptavidin molecules.The streptavidin membrane (the thickness or 500urn) was used toimmobilize anti-human chorionic gonadotrophin antibody (Q hCG). The number orQbCG in the membrane was about 1011 per mm2 of the membrane surface. The membrane was used as a sensing membrane or optical immunosensor (Mach-Zehnder interferomter). It had a good response at the concentration or 10 afl/L hCG. |
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| Keywords: | :immobilization or protein biotin-avidin system sensingmembrane biosensor optical immunosensor |
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