Removal of Leukocytes from Whole Blood and Erythrocyte Suspensions by Filtration through Cotton Wool |
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| Authors: | P. Diepenhorst R. Sprokholt and H. K. Prins |
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| Affiliation: | Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam |
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| Abstract: | Abstract. (1) Sterile filtration of whole blood or blood cell suspensions in saline through columns of tightly packed cotton wool results in removal of over 95% of the leukocytes. (2) From whole blood, thrombocytes are inefficiently removed by the filtration procedure; filtration of blood cell suspensions in saline results in a better removal. (3) A system is designed by which filtration of 500 ml portions can be performed in less than 1 h at room temperature. (4) Red cell recovery with this system is over 90%. (5) Neither pyrogen tests nor toxicity tests did reveal the elution of harmful substances from properly prepared columns. |
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