| 胰岛素对体外培养的视网膜Müller细胞VEGF变化影响的研究 |
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| 引用本文： | 王越晖 宋鄂 等. 胰岛素对体外培养的视网膜Müller细胞VEGF变化影响的研究[J]. 中国病理生理杂志, 2002, 18(9): 1066-1068 |
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| 作者姓名： | 王越晖 宋鄂 等 |
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| 作者单位： | 1. 吉林大学基础医学院病理教研室;
2. 吉林大学第一临床医院眼科, 吉林长春130021 |
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| 基金项目： | 吉林省科学基金资助项目 (No.990 5 75 - 5 ) |
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| 摘 要： | 目的:观察在不同浓度胰岛素条件下, 体外培养的兔视网膜M櫣ller细胞的血管内皮生长因子(vascularendothelialgrowthfactor, VEGF)表达的变化。方法:采用免疫细胞化学法和ELISA方法, 定性定量测定不同浓度胰岛素条件下体外培养的兔视网膜Müller细胞VEGF的表达水平的升高可能是胰岛素能明显增强VEGF的表达。结论:高浓度胰岛素可增强M櫣ller细胞VEGF的表达, 这种VEGF表达水平的升高可能是胰岛素促进糖尿病视网膜病变的因素之一。
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| 关 键 词： | 胰岛素 内皮生长因子 Müller细胞 糖尿病视网膜病 |
| 文章编号： | 1000-4718(2002)09-1066-03 |
| 收稿时间： | 2002-04-09 |
| 修稿时间： | 2002-04-09 |
Effects of insulin on VEGF expression in cultured retinal Müller cells |
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| WANG Yue-hui ,SONG E ,WANG Xin-rui ,ZHU Xiang-hong ,WU Jia-xiang. Effects of insulin on VEGF expression in cultured retinal Müller cells[J]. Chinese Journal of Pathophysiology, 2002, 18(9): 1066-1068 |
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| Authors: | WANG Yue-hui SONG E WANG Xin-rui ZHU Xiang-hong WU Jia-xiang |
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| Affiliation: | Department of Pathology and Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China |
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| Abstract: | AIM: To investigate the effect of insulin on the expression of vascular endothelial growth factor(VEGF) in cultured rabbit retinal Müller cells. METHODS: Immunocytochemical method and ELISA were used to assay the change of VEGF expression in cultured Müller cells in vitro at different insulin concentrations in qualitative and quantitative ways. RESULTS: VEGF expression in retinal Müller cells was enhanced markedly by insulin. CONCLUSION: Insulin at high concentration enhances VEGF expression in cultured Müller cells, which may be one of factors that insulin accelerates the progress of diabetic retinopathy. |
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| Keywords: | Insulin Endothelial growth factors Müller cells Diabetic retinopathy |
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