人生长激素对结肠癌细胞株细胞周期动力学的影响

目的：了解人生长激素（ｈＧＨ）对于结肠癌细胞有无促增殖和分化作用。方法：取对数生长期的人结肠癌细胞株ＬＯＶＯ，以顺铂和（或）不同浓度的ｈＧＨ体外培养，２４ｈ后以流式细胞增殖周期及细胞凋亡等指标。结果：同时加顺铂和ｈＧＨ培养细胞，癌细胞群在Ｇ２－Ｍ期发生阻滞，细胞凋亡率增加，Ｓ期细胞数率非常显著地降低；加ｈＧＨ１００ｎｇ／ｍｌ组Ｇ０－Ｇ１期九率降低，Ｓ期百分率增高。结论：ＬＯＶＯ细胞表面可能有ｈＧＨ

Effect of human growth hormone on cell cycle kinetics in LOVO cells in vitro

Objectives:To study effect of human growth hormone (hGH) on cycle kinetics and apoptosis of human calonic cancer cell line,LOVO cells. Methods:LOVO cells were cultured for 24 hr in 1640 with or without DDP,but with hGH at a concentration of 50,100 or 200 ng/ml.Cells undergoing apoptosis and differentiation were determined by flow cytometry(FCM). Results:hGH had significant effect on LOVO cells cycle rate,percent G 0~G 1 phase dropped( P < 0.05 ),percent S phase increased( P <0.01).Addition of hGH and DDP to the culture medium raised cell rates of G 2~M phase,while apoptosis of LOVO cells increased. Conclusions:LOVO cells might have expression for the GH receptor.In vitro hGH might induce differentiation of cells LOVO and promote cell cycle kinetics.DDP could suppress the stimulatory effect of hGH on percent S phase,and increase apoptosis of LOVO cells.