[3H]5-HT binding in post-mortem human cerebral cortex: methodological considerations |
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| Authors: | S. C. Cheetham R. W. Horton |
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| Affiliation: | (1) Present address: Department of Pharmacology and Clinical Pharmacology, St. George's Hospital Medical School, London, UK |
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| Abstract: | Summary The effects of different membrane preparations and assay conditions on [3H]5-HT binding to post-mortem human cortical tissue was studied. Optimal binding necessitated thorough removal of endogenous 5-HT and this was achieved either by hypotonic lysis or by preincubation of the membranes at 37 C. Calcium chloride (4 mM) increased specific [3H]5-HT binding. The further addition of ascorbic acid (5.7 mM) or ascorbic acid and clorgyline (10  M) reduced specific [3H]5-HT binding. |
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| Keywords: | [3H]5-HT binding human cortex calcium chloride ascorbic acid clorgyline |
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