高胰岛素对人内皮细胞分泌前列环素和内皮素-1的影响

目的：了解高胰岛素对人内皮细胞分泌前列环素（PGI2）和内皮素-1（ET-1）的影响.方法：采用酶消化法收集人脐静脉内皮细胞,经原代培养后随机分为4组：正常对照组,高胰岛素组,正常＋谷胱甘肽组,高胰岛素＋谷胱甘肽组;放免法测定4种培养基培养24 h,48 h,72 h时培养基中的PGI2和ET-1含量.结果：①高胰岛素组较正常对照组PGI2显著下降（P＜0.01）,ET-1显著升高（P＜0.01）;②高胰岛素＋谷胱甘肽组较高胰岛素组PGI2显著升高（P＜0.05）和ET-1显著下降（P＜0.05）.结论：高胰岛素对血管内皮细胞有损伤作用,还原型谷胱甘肽可减轻这种损伤.

Influence of high insulin on human vascular endothelial cells secreting prostacyclin and endothelin-1

Aim: To investigate the influence of high insulin on human umbilical vein endothelial cells(HUVEC)secreting prostacyclin(PGI2) and endothelin-1(ET-1).Methods: After primary culture,the HUVEC were allocated into four groups:normal control group, normal control with glutathione group, high insulin group, and high insulin with glutathione group; the medium PGI2 and ET GA 1 in the 4 groups which were cultured for 24 h,48 h, and 72 h were assayed using radioimmunoassay. Results: The content of PGI2 in the high insulin group was lower, and the content of ET-1 was higher compared with normal control group (P< 0.01); the content of PGI2 in the high insulin with glutathione group was higher and the content of ET-1 was lower(P<0.05)compared with the high insulin group. Conclusion: High insulin can injure vascular endothelial cells and reduced glutathione can ameliorate the endothelial dysfunction.