甲状腺素对梗阻性黄疸大鼠肝脏保护作用的研究

目的探讨外源性甲状腺素对梗阻性黄疸大鼠肝脏保护作用及其机制。方法24只SD大鼠随机分为对照组、模型组及干预组，模型组及干预组采用胆总管结扎法建立梗阻性黄疸大鼠模型，干预组给予外源性甲状腺素干预20μg／（kg·d）]，14d后采用放射免疫法检测各组大鼠血清甲状腺激素水平、酶联免疫吸附法检测血清白蛋白（ALB）、谷丙转氨酶（ALT）、总胆红素（TBIL）、直接胆红素（DBIL）、谷草转氨酶（AST）、凝血酶原活度（PA）水平。结果造模14d后干预组大鼠游离甲状腺素FT3、FT4水平低于对照组（P〈0．05），高于模型组（P〈0．05），干预组及模型组促甲状腺激素（TSH）水平低于对照组（P〈0．05），干预组大鼠血清ALT、TBIL、DBIL、AST水平低于模型组（P〈0．05），高于对照组（P〈0．05），ALB、PA水平高于模型组（P〈0．05），低于对照组（P〈0．05）。结论外源性甲状腺素能够纠正梗阻性黄疸大鼠体内甲状腺素异常，发挥对肝脏的保护作用。

Study on the protective effects of thyroxine for hepatic fibrosis on rats with obstructive jaundice

Objective To investigate the protection and mechanism of ecyogentic thyroxine for hepatic fibrosis rats on with obstructive jaundice.Methods 24 SD rats were randomly divided into control group(CG),medol group(MG) and intervention group(IG).MG group and IG group were established by ligating-mutilation bile duct in MG and IG,ecyogentic thyroxine were all taken in IG group with 20 μg/(kg·d),levels of thyroid hormone were detected with radioimmunity,levels of ALB,ALT,TBIL,DBIL,AST and PA were detected with enzyme linked immunosorbent assay after 14 days.Results After 14 days,levels of FT3 and FT4 in serum were lower in IG than in CG(P < 0.05),and were higher in IG than in MG(P < 0.05),levels of TSH were lower in MG and IG than in CG(P < 0.05),levels of ALT,TBIL,DBIL,AST were lower in IG than in MG(P < 0.05),and were higher than in CG(P < 0.05),levels of ALB and PA were higher in IG than in MG(P < 0.05),and were lower than in CG(P < 0.05).Conclusion Ecyogentic thyroxine can improve abnormality of thyroxine metabolism in rats with obstructive jaundice and protect liver.