外源性胰岛素样生长因子-1对脓毒血症大鼠肺保护的作用

目的观察外源性胰岛素样生长因子-1对大鼠脓毒血症引起急性肺损伤的肺保护作用。方法选择Wistar大鼠40只,随机分为4组（每组各10只）：对照组,脓毒血症组,IGF-1治疗1组和治疗2组（50μg/kg和100μg/kg）。采用颈静脉注射脂多糖（LPS,15 mg/kg）制作脓毒血症大鼠ALI模型。注射LPS 12 h后,测定各组大鼠血清和支气管肺泡灌洗液IL-6和IL-8浓度。结果与对照组比较,脓毒血症组大鼠血清IL-6浓度（25.5±3.71）vs（14.6±2.34）ng/mL]和支气管肺泡灌洗液IL-6浓度（17.9±2.56）vs（9.1±1.19）ng/mL]均高于对照组,也高于IGF-1治疗组（均P〈0.05）,同时脓毒血症组在血清IL-8（2.03±0.15）vs（1.62±0.13）ng/mL]和支气管肺泡灌洗液的IL-8浓度（1.35±0.37）vs（0.98±0.11）ng/mL]均高于对照组和IGF-1治疗组（均P〈0.05）。IGF-1治疗2组大鼠血清和支气管肺泡灌洗液IL-6（17.3±4.59）vs（17.3±4.59）,（11.4±2.93）vs（13.6±2.71）ng/mL]和IL-8（1.73±0.11）vs（1.87±0.26）,（0.99±0.39）vs（1.09±0.43）ng/mL]浓度均低于IGF-1治疗1组（P〈0.05）。结论外源性IGF-1对脓毒血症大鼠引起的急性肺损伤具有保护作用,且随着剂量的加大保护作用更明显。

Protective effects of insulin-like growth factor-1 on sepsis induced acute lung injury in rats

Objective To study protective effects of insulin-like growth factor-1(IGF-1) on sepsis induced acute lung injury(ALI) in rats.Methods Forty male SD rats were randomly divided to four groups of control group,model group,IGF-1 treating groups(IGF-1 treating group 1 and IGF-1 treating group 2 with IGF-1 of 50 μg/kg and 100 μg/kg,respectively).The animal model of sepsis induced ALI was established by infusing lipopolysaccharide(LPS) 15 mg/kg via jugular vein.At the 12 h after establishment of models,serum and bronchoalveolar lavage fluid(BALF) IL-6 and IL-8 were determined.Results Compared with normal group,the serum and BALF IL-6 (25.5±3.71) vs(14.6±2.34),(17.9±2.56) vs(9.1±1.19) ng/mL] and IL-8(2.03±0.15) vs(1.62±0.13),(1.35±0.37) vs(0.98±0.11) ng/mL] were increased in model group and IGF-1 treating groups(P < 0.05).Compared with IGF-1 treating group 2,the serum and BALF IL-6 (17.3±4.59) vs(17.3±4.59),(11.4±2.93) vs(13.6±2.71) ng/mL] and IL-8 (1.73±0.11) vs(1.87±0.26),(0.99±0.39) vs(1.09±0.43) ng/mL] were lowered in IGF-1 treating group 1(P < 0.05).Conclusion Exogenous IGF-1 has the protection effect to acute lung injury caused by sepsis rats,and with the increase of dose protection,the effect will be more obvious.