HPLC法测定复方选净散中水杨酸和苯甲酸的含量

目的采用HPLC法测定复方选净散中水杨酸和苯甲酸的含量。方法采用Diamonsil C18色谱柱（250mm×4．6mm，5μm），流动相为甲醇-0．05mol／L磷酸氢二钠溶液（用磷酸调pH至3．9）（55：45），柱温为30℃，流速为0．8mL／min，检测波长233am，以外标法峰面积定量。结果水杨酸、苯甲酸分别在0．0359-0．2993mg／mL（r=0．9998），0．0358～0．2980mg／mL（r=0．9993）范围内有良好的线性关系，平均回收率分别为100．14％（RSD=0．77％）和99．95％（RSD=0．95％）（n=9）。结论本方法简便、准确，可快速测定复方选净散中水杨酸和苯甲酸的含量，可为质量控制提供依据。

Determination of Salicylic acid and Benzoic acid in Fufangxuanjingsan by HPLC

Objective To establish the HPLC method for the content determination of Salicylic acid and benzoic acid in Fufangxuanjingsan.Methods Diamonsil-C18 column(250 mm × 4.6 mm,5 μm) was used with the mobile phase of amixture of methanol and 0.05 mol/L KH2PO4(55∶45)(adjusted to pH 3.9 with H3PO4) as mobile phase;the flow rate was 0.8 mL/min;the column temperature was 30℃;the detection wavelength UV 233 nm.Results The calibration curves of Salicylic acid and Benzoic acid was linear relationship in the range of 0.035 9-0.299 3 mg/mL(r = 0.999 8),0.035 8-0.298 0 mg/mL(r = 0.999 3).The average recoveries(n = 9) was 100.14%(RSD=0.77%) and 99.95%(RSD=0.95%) respectively.Conclusion The established method is simple,quick and accurate for the simultaneous separation and determination of Salicylic acid and Benzoic acid in Fufangxuanjingsan.