决明子降血脂有效部位及其量效关系的实验研究

目的研究决明子降血脂的有效部位及其量效关系。方法选用CO超临界、系统溶媒(石油2醚、乙酸乙酯、70%乙醇、水)和水提醇沉的方法分别对决明子进行提取,观察各提取物对腹腔注射蛋黄乳液致高血脂症模型小鼠血清总胆固醇及甘油三酯的影响,初步明确决明子降血脂的有效活性部位,并对该有效部位进行量效关系的评定。结果决明子乙酸乙酯提取物和水提取物均有显著的降低血清总胆固醇和甘油三酯的作用,其中乙酸乙酯提取物的降脂效果优于水提取物,其在0.75～1.00g·kg-1·d-1时降脂效果最佳;决明子70%乙醇提取物和水提醇沉物虽也有一定的降血脂作用,但与对照组相比差异无统计学意义。结论决明子乙酸乙酯提取物为决明子降血脂的有效活性部位,其有效剂量范围在0.50～1.25g·kg-1·d-1之间。

Experimental study on the active situs of fetid cassia seed to reduce blood lipid and their dose-effect relation

Objective To probe into the active situs of fetid cassia seed (semen cassiae) reducing blood lipid and research the best dose-effect relation of the active situs. Methods Using supercritical fluid extraction(SFE),systematic solvents (petroleum benzin,ethyl acetate,70%alcohol and water) and alcohol-precipitation after water-extraction to extract the fetid cassia seed;the effects of different extracts on reducing the serum levels of total cholesterol and triglyceride of the hyperlipemic models were observed,which were established through celiac injection of 75%egg-yolk emulsion;a preliminary clarification of the active situs of fetid cassia seed reducing blood lipid was also made;it's dose- effect relation was studied and assessed. Results The fetid cassia seed ethyl acetate extraction and the fetid cassia seed water extract both have obvious effect on reducing the serum total cholesterol and triglyceride;morever,the ethyl acetate extract reducing blood lipid was better than the water extract,its best dose was 0.75～1.00 g·kg-1·d-1 .Additionally, though the fetid cassia seed 70% alcohol extract and the alcohol precipitate from water extract had effect on reducing blood lipid,they both hadn′t remarkable different from the control group. Conclusion The fetid cassia seed ethyl acetate extract is the active situs of fetid cassia seed reducing blood lipid;its effective dose ranges 0.50～1.25 g·kg-1·d-1.