| 随机扩增多态性DNA技术用于鼠疫耶尔森氏菌基因分型的研究 |
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| 引用本文： | 黄芳,俞东征,海荣,蔡虹.随机扩增多态性DNA技术用于鼠疫耶尔森氏菌基因分型的研究[J].中华流行病学杂志,2000,21(6):424-426. |
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| 作者姓名： | 黄芳 俞东征 海荣 蔡虹 |
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| 作者单位： | 中国预防医学科学院流行病学微生物学研究所鼠疫研究室,北京,102206 |
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| 摘 要： | 目的 对来自全国不同疫源地、不同生态型的１０３株鼠疫耶尔森氏菌进行基因分型研究。方法 用随机扩增多态性ＤＮＡ技术（ＲＡＰＤ）。结果 将鼠疫耶尔森氏菌分成两种基因型别：全国大部分菌株为ＲＡＰＤ－１型，青海省境内的大部分菌株为ＲＡＰＤ－２型。结论 不同生态型的鼠疫耶尔森氏菌基因结构存在差异，为鼠疫耶尔森氏菌的基础研究和防治提供依据。
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| 关 键 词： | 鼠疫耶尔森氏菌 随机扩增多态性 DNA技术 |
| 收稿时间： | 2000/6/12 0:00:00 |
| 修稿时间： | 2000-06-12 |
Study on the application of randomamplified polymorphic DNA in Yersinia pestis genotyping |
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| HUANG Fang,YU Dongzheng,HAI Rong and CAI Hong.Study on the application of randomamplified polymorphic DNA in Yersinia pestis genotyping[J].Chinese Journal of Epidemiology,2000,21(6):424-426. |
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| Authors: | HUANG Fang YU Dongzheng HAI Rong and CAI Hong |
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| Institution: | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206, China. |
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| Abstract: | OBJECTIVE: To study the type of 103 Y. pestis strains that isolated from different plague foci and different ecological types. METHOD: Random amplified polymorphic DNA (RAPD) was used. RESULTS: Y. pestis were divided into two RAPD types: most strains that isolated from elsewhere in the country belonged to RAPD-1 type, while most strains that isolated from Qinghai province were RAPD-2 type. CONCLUSION: The genome structures of different ecotype Y. pestis were different providing the foundation of further research and prevention of plague. |
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| Keywords: | Yersinia pestis Random amplified polymorphic DNA |
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