慢性粒细胞白血病融合基因与中医分型探讨

目的：探讨慢性粒细胞白血病（CML）患者BCR／ABL融合基因表达与中医辨证分型的关系。方法：采用筑巢式RT—PCR方法，扩增30例CML患者BCR／ABL融合基因，从分子生物学角度探讨其与中医分型的关系。结果：气阴两虚型与瘀血痰结型患者BCR／ABL基因阳性率明显高于毒热炽盛型（P〈0．01），经过治疗后，瘀血痰结型BCR／ABL基因阳性率高于气阴两虚型（P〈0．05）。结论：90％以上的CML患者都具有BCR／ABL融合基因的表达，该融合基因是实验室诊断指标之一，其表达与变化为指导CML中医辨证诊治提供了客观指标。

Study on Relationship between of Fusion Gene and TCM Syndrome Classification in the Patient of Chronic Myelogenous Leukemia

Objective:To study on relationship between of BCR/ABL fusion gene expression and TCM syndrome classification in the patient of chronic myelogenous leukemia(CML).Methods:Nest-building RT-PCR method was used for amplification of BCR/ABL fusion gene in 30 cases of CML,so as to explore the relationship between gene and TCM syndrome classification from the perspective of molecular biology.Results:The positive rate of BCR/ABL gene in the patient with both qi and yin deficiency,and the patient of intermingled phlegm and blood stasis was significantly higher than that in the patient of intense noxious heat type(P<0.01).After treatment,the positive rate of BCR/ABL gene in the patient of intermingled phlegm and blood stasis was higher than that in the patient with both qi and yin deficiency(P<0.05).Conclusion:Over 90% patients of CML have expression of BCR/ABL fusion gene,which is a laboratory index for diagnosis of CML,and its expression and changes are an objective index for guiding diagnosis and treatment of CML.