BCL—2蛋白在癫痫过程中海马细胞内的变化

癫痫全面性强直阵挛发作（ＧＴＣＳ）可诱导脑细胞的损伤与修复过程。ＢＣＬ—２蛋白是具有介导多种细胞外信号保护细胞存活的重要的细胞浆蛋白之一。应用流式细胞免疫荧光技术对马桑内酯（ＣＬ）致痫大鼠海马细胞中ＢＣＬ—２蛋白进行检测。结果显示：ＣＬ诱导癫痫发作海马细胞中ＢＣＬ—２蛋白表达增高，发作６小时以内荧光指数（ＦＩ）（１．１２９±０．０４７，ｎ＝７）明显高于对照组（０．９９９±０．０９６，ｎ＝９），Ｐ＜０．００５，６～２４小时组（１．０２６±０．０６５，ｎ＝３）及２４小时以后组（０．９８２±０．０４３，ｎ＝６）ＦＩ回落，Ｐ＞０．０５。提示这种ＢＣＬ—２变化可能对损伤和凋亡细胞具有保护作用。

BCL-2 Protein Changes With in the Hippocampus Cells in Coriaria Lacto-induced GTC Seizure in Rats

Generalized tonic-clonic seizure (GTCS) may induce the injury and repair of the brain cells. The BCL- 2 protein is a key member of a growing family of cytoplasmic proteins that modulate the response of many type of cells to diverse extracellular signals by which it may promote the survival of the cells.By using flow cytometry and immunofluorescence techniques, the changes of BCL-2 protein of the hippocampus cells in rats after the seizures induced by Coriaria Lactone(CL)were primarily studied.Results:The expression of BCL- 2 in hippocampus cell of epileptic episodes in rat was increased by the inducement of CL. The fluorescence index(FI)of the cells within 6 hours following CL induced (1. 129±0. 047,mean±s,n=7)was significantly higher than that of normal control group (0.999±陈.096, n =9),P<0.005;Then the FI of BCL- 2 was decreased between 6 - 24 hours inducemen of CL(1.026 ±0.065,n =3)and in the group after induced by CL for 24 hours,the FI were also decreased (0.982 ±0.043,n=6). But the FI in the latter two groups was not significantly different from that in control group, P>0. 05.The results suggested that such a change of BCL-2 protein in the hippocampus cells may protect the cells from injuring and apoptosing.