康脑灵冲剂对高脂合并脑缺血大鼠自由基代谢的影响

目的 :观察康脑灵冲剂对高血脂合并脑缺血模型大鼠体内血浆脂蛋白、自由基代谢异常的影响。方法 :建立高脂餐饮食加双侧颈动脉结扎形成急性实验性不完全性脑缺血模型 ,给模型大鼠服用复方丹参片、康脑灵冲剂治疗 ,用放免法、光化学扩增法 [1 ]、硫代巴比妥酸法 [2 ]检测 TXB2 、6 - keto- PGF1 α、ET、SOD、MDA、L DL- C、HDL-C、apo A- I、apo B、NO的含量。结果 :用药组与模型组大鼠比较血浆 L DL- C、apo B含量明显降低 ,HDL- C、apo A- I含量增多 ,ET、TXB2 减少 ,6 - keto- PGF1α增高 ,NO含量增多 ,SOD活力升高 ,MDA含量减少 ,有显著性差异。结论 :康脑灵冲剂具有活血化瘀、抗氧化、抗脑缺血缺氧作用 ,使内皮细胞合成和释放内皮素 (ET)等物质减少 ,减轻血管内皮损伤 ,从而可防止动脉硬化、血管性疾病及其他并发症的发生。

EFFECTS OF KANGNAOLINGON FREE RADICAL METBOLISM IN HYPERLIPOPROTEINEMIA MICE COMBINED WITH CEREBRAL ISCHEMIA

Objective:To observe the effects of Kangnaoling granule on metabolism of plasma lipoprotein and radical in hyperlipoproteinemia mice combined with cerebral ischemia.Methods:A rat model of acute cerebral ischemia was established through being fed with high fat food and ligated double carotid arteries.Some indexes in rats were determined by radio immunity,photo chemical expansion,Thiobarbiturate.Results:Compared with the model group,the contents of plasma LDL C,apoB in the observation group decreased,while those of HDL C,apoA increased.The serum levels of ET,TXB 2 in the observation group were decreased,but those of 6 keto PGF 1α and NO increased and the activity of SOD increased,which were more obvious than those of model group.Conclusion:Kangnaoling granule has the functions of antioxydation,resisting thrombus and cerebral ischemia.It can also reduce the level of ET,relieve hurts on vessel endothelium,therefore can inhibit occurrence of arteriosclerosis and other complications.