镰刀菌T-2毒素对人急性早幼粒白血病细胞HL60增殖与凋亡的影响

目的研究镰刀菌属真菌产生的倍半萜烯类化合物T-2毒素对体外培养急性早幼粒白血病细胞HL60生长抑制与凋亡诱导的作用。方法体外培养的HL60细胞经0、4、8、16和32μg/ml的T-2毒素处理48h,以MTT法检测T-2毒素对HL60细胞的生长抑制作用;吉姆萨染色观察细胞凋亡形态;流式细胞术(FCM)检测凋亡率;FCM及Western blot检测凋亡相关蛋白Bax、Bcl-2的表达情况。结果 MTT显示T-2毒素对HL60细胞的增殖具有抑制作用,并呈剂量依赖关系。形态学观察显示早幼粒白血病细胞出现明显凋亡形态特征。FCM定量检测表明,T-2毒素各处理组细胞凋亡率均高于对照组,并随T-2毒素浓度升高而增加。FCM和Western blot结果显示,T-2毒素处理后HL60细胞Bax蛋白表达上调,Bcl-2蛋白表达下降。结论T-2毒素可剂量依赖地抑制体外培养的HL60细胞增殖并诱导凋亡。

Effects of T-2 toxin of Fusarium on proliferation and apoptosis of human acute promyelocytic leukemia cellline HL60

Objective To study the effects of sesquiterpenes compounds T-2 toxin produced by Fusarium fungi on proliferation and apoptosis of acute promyelocytic leukemia cellline HL60.Methods HL60 cells were treated with T-2 toxin(0,4,8,16 and 32 μg/ml) for 48 h,and then cells were harvested for the studies of growth inhibition with MTT,Cell morphology with Giemsa staining,apoptosis and the expression of Bax and Bcl-2 with flowcytometry(FCM) and Western blot.Results MTT results showed that T-2 toxin inhibited HL60 proliferation on dose-dependent.Morphological observation showed significant apoptotic morphological characteristics.FCM results showed that the apoptosis rates of HL60 cells in T-2 toxin treatment groups were higher than that in control,and positive dose-effectc orrelations could be found.FCM and Western blot results showed that the expression of Bax was increased while that of Bcl-2 was decreased in T-2 toxin treatment groups of HL60.Conclusion The results suggested that T-2 toxin could inhibite proliferation and induce apoptosis of HL60 cells in vitro in a dose-dependent manner.