微波消解-电感耦合等离子体质谱法测定人乳中24种矿物质含量

目的用微波消解-电感耦合等离子体质谱法测定人乳中24种矿物质,以评价人乳的矿物质水平。方法样品经微波消解后,用电感耦合等离子质谱仪测定24种元素。以加标实验和美国NIST的标准参考物质1849a、1568a考察方法的准确性与重现性。结果本实验检测的人乳中钠、镁、磷、钾、钙、铝、铬、砷、硒、铁、锌、锰、铜、钼、钒、钴、镍、镓、镉、银、锶、铯、钡、铅的水平依次为34.97～415.83mg/kg,19.00～39.52mg/kg,102.13～274.53mg/kg,351.19～713.99mg/kg,180.08～349.64mg/kg,0.06～0.44mg/kg,0.9～7.37μg/kg,0.92～2.72μg/kg,0.20～21.15μg/kg,0.10～0.70mg/kg,0.56～3.25mg/kg,3.00～16.12μg/kg,62.16～591.69μg/kg,0.02～6.91μg/kg,5.99～13.70μg/kg,0.07～2.11μg/kg,0.77～209.26μg/kg,0.005～0.28μg/kg,0.02～0.23μg/kg,0.02～0.71μg/kg,36.89～132.26μg/kg,0.01～4.72μg/kg,0.83～28.16μg/kg,2.5～5.3μg/kg。该水平与国内外其他文献检测的水平相近。结论本文检测了人乳中24种矿物质水平,具有较高的准确性和较好的重现性,可以为全面了解人乳矿物质水平提供部分数据支持。

Determination of 24 minerals in human milk by inductively coupled plasma mass spectrometry with microwave digestion

Objective To determine the levels of 24 minerals in human milk by inductively coupled plasma mass spectrometry with microwave digestion.Methods The samples were digested by microwave.The contents of minerals were determined by inductively coupled plasma mass spectrometry.The standard reference minerals of 1849a and 1568a from National Institute of Science and Technology were used for quality control.The accuracy and reproduability for this method were evaluated with mix standards and 1849a and 1568a standard reference materals.Results The ranges of the levels of sodium,magnesium,phosphorus,potassium,calcium,aluminum,chromium,arsenic,selenium,iron,zinc,manganese,copper,molybdenum,vanadium,cobalt,nickel,gallium,cadmium,silver,strontium,cesium,barium,lead in human milk was 34.97-415.83mg/kg,19.00-39.52mg/kg,102.13-274.53mg/kg,351.19-713.99mg/kg,180.08-349.64mg/kg,0.06-0.44mg/kg,0.9-7.37μg/kg,0.92-2.72μg/kg,0.20-21.15μg/kg,0.10-0.70mg/kg,0.56-3.25mg/kg,3.00-16.12μg/kg,62.16-591.69μg/kg,0.02-6.91μg/kg,5.99-13.70μg/kg,0.07-2.11μg/kg,0.77-209.26μg/kg,0.005-0.28μg/kg,0.02-0.23μg/kg,0.02-0.71μg/kg,36.89-132.26μg/kg,0.01-4.72μg/kg,0.83-28.16μg/kg,2.5-5.3μg/kg,respectively.The levels of minerals in human milk in present study were consisted with other similar studies.Conclusion The experiment examined the levels of minerals in human milk satisfactorily.The method has high accuracy and good reproducibility,which could be used for understanding the levels of minerals in human milk.