生命早期经饲料补充维生素D对链脲菌素致小鼠糖尿病的影响

目的探讨生命早期经饲料补充维生素D对链脲菌素作用小鼠空腹血糖和胰岛素水平,糖尿病发病率,以及链脲菌素致胰岛损伤的影响。方法 C57BL/6鼠离乳后分别饲喂标准饲料和补充维生素D(VD)饲料9周后注射链脲菌素(STZ),继续喂养4周,注射STZ后每周末测定空腹血糖,计算糖尿病发病率,4周后测血清胰岛素水平,并采用HE染色法观察胰腺形态学变化。结果①生命早期补充VD能降低STZ作用小鼠的空腹血糖水平,并以补充高剂量VD的降糖效果最好,其在注射STZ后4周血糖值明显低于糖尿病模型组(P<0.01);②生命早期补充VD降低STZ致小鼠糖尿病发病率,并以补充高剂量VD的干预效果最好,其在注射STZ后4周时糖尿病发病率为0%,完全抑制糖尿病的发病(P<0.01);③生命早期补充高剂量和中剂量VD明显上调STZ作用小鼠的血清胰岛素水平(P<0.05),并以补充高剂量VD的上调作用最显著(P<0.01)。④生命早期补充高剂量和中剂量VD对STZ致胰岛损伤有明显保护作用,且以补充高剂量VD的保护作用最好。结论生命早期补充一定量VD能降低STZ作用小鼠的血糖水平和糖尿病发病率,上调血清胰岛素水平,改善STZ对胰岛细胞的损伤,且均以补充高剂量VD的作用最显著。

Impact of oral vitamin D supplementation in early life on diabetic mice induced by streptozotocin

Objective To evaluate the effect of oral vitamin D supplementation in early life on blood glucose,insulin content,diabetes incidence,and histomorphology in pancreatic islet induced by streptozotocin.Methods Three-week-old C57BL/6 mice were given either control diet(American Institute of Nutrition-93G),or three different dose of vitamin D-supplemented diet.Nine weeks after dietary intervention,C57BL/6 mice were treated with streptozotocin i.p.for 5 consecutive days.After injection of STZ,The fasting blood glucose and diabetes incidence was tested once a week.The insulin content,histomorphology in pancreatic islets was conducted at the end of experiments.Results ①Vitamin D supplementation in early life can decrease the fasting blood glucose values induced by STZ,and the decreases effect of high dose vitamin D-supplemented group is the most significant(P<0.01).②Vitamin D supplementation in early life can prevent diabetes incidence,and the decreases effect of high dose vitamin D-supplemented group is the most significant,fully suppress the onset of diabetes about four weeks later after injected by STZ(P<0.01).③As compared to the control group,insulin content in medium and high dose vitamin D-supplemented groups were significantly up-regulated after injection of STZ(P<0.05),and the effect of high dose vitamin D-supplemented group is the most significant.④The damage of pancreatic islets induced by STZ was clearly restored in medium and high dose vitamin D-supplemented groups,and effect of high dose vitaminD-supplemented group is the most significant.Conclusion Oral vitamin D supplementation in early life can decrease the fasting blood glucose values,prevent diabetes incidence,up-regulate insulin content,restored the damage of pancreatic islets induced by STZ,and effects of high dose vitamin D-supplementde group are all the most significant.