川崎病患儿血清IL-3、IL-6、TPO水平变化研究

目的研究川崎病（KD）患儿血清白细胞介素-3（IL-3）、白细胞介素-6（IL-6）、促血小板生成素（TPO）水平变化，探讨其与冠脉损害（CAL）的关系。方法采用双抗体夹心酶联免疫吸附实验（ELISA），分别测定正常对照组与KD患儿急性期、治疗后恢复期血清IL-3、IL-6、TPO水平。结果①KD患儿急性期IL-3水平为248．93±126．00pg／ml，与治疗后恢复期的148．20±45．26pg／ml相比，P〈0．001；与正常对照组的115．62±43．72pg／ml相比，P〈0．05。②急性期IL-6水平为230．20±118．52pg／ml，与治疗后恢复期的98．47±25．12pg／ml相比，P〈0．001，与正常对照组68．33±22．63pg／ml相比，P〈0．01。⑧急性期TPO水平为337．5±117．77pg／ml，与治疗后恢复期120．03±46．69pg／ml相比，P〈0．001，与正常对照组的109．12±53．89Pg／ml相比，P〈0．01。④35例KD患儿中7例伴发CAL，28例无冠脉损害（NCAL）。CAL组IL-3水平为276．51±119．20pg／ml，与NCAL组的213．58±108．75pg／ml相比P〉0．05；CAL组IL-6水平为262．67±119．20pg／ml，与NCAL组的202．32±93．36pg／ml相比P〉0．05；CAL组TPO水平为406．85±138．78pg／ml，与NCAL组的287．62±123．63pg／ml相比，P〈0．05。结论IL-3、IL-6、TPO在KD急性期水平增高，恢复期水平下降。提示：KD急性期炎性因子水平增加。KD患儿伴CAL组的TPO水平明显增高，提示TPO的水平增高可能与冠脉损害有关，可做为KD患儿伴CAL的预测指标。

Changes of the Serum IL-3,IL-6,TPO Levels in Children with Kawasaki Disease

Objective To study the serum levels of interleukin 3 （IL-3）, interleukin 6 （IL-6） and TPO （thrombopoietin） in children with Kawasaki disease （KD） and to explore their relationships with coronary artery lesions（CAL）. Methods 35 KD children and 25 healthy children as control group were enrolled in this study. The serum levels of IL-3, IL-6, TPO were detected by enzyme-linked immunosorbent assay （ELISA）. Results ①The serum level of IL-3 in children with KD in acute phase was 248.93 ± 126.00 pg/ml, significantly higher than that in convalescent phase （148.20±45.26pg/ml） and than that in control group （115.26±43.72pg/ml） （P 〈 0. 001 and 0.05 ）, while it had no statistical difference between the children in convalescent phase and in control group （ P 〉 0.05 ）. ② The serum level of IL-6 in children with KD in acute phase was 230.20±118.52pg/ml, significantly higher than that in convalescent phase （98.47±25.12 pg/ml） and than that in control group （68.33±22.63 pg/ml） （P〈0. 001 and 0.01 ）, while it had no statistical difference between the children in convalescent phase and in control group （P〉0.05）. ③ The TPO level in patients in acute phase was 337.50 ± 117.77 pg/ml, significantly higher than that in convales-cent phase （120.03±46.69 pg/ml） and than that in control group （ 109.12±53.89 pg/ml） （ P 〈0. 001 ）, while it had no statistical difference Between the children in convalescent phase and in control group （P〉0.05）.④between 7 CAL and 28 NCAL（ non-coronary artery lesion） KD patients, the serum level of IL-3 has no significant difference （276.51±119.20 pg/ml and 213.58±108.75 pg/ml, P 〉0.05）. The level of IL-6 has also no significant difference（262.67±119.20 pg/ml and 202.32±93.36 pg/ml, P〉0.05）. While the TPO level was significantly higher in KD with CAL than that NCAL（406.8±138.78pg/ml and 287.62±123.63 pg/ml, P 〈0.05）. Conclusion IL-3 ,IL-6,TPO which serum levels increased in KD patients wi