| 新型生物活性复合材料RGD-PLGA/HA体外细胞相容性的实验研究 |
| |
| 引用本文： | 张士杰,陈飞,蒋欣泉.新型生物活性复合材料RGD-PLGA/HA体外细胞相容性的实验研究[J].山东大学学报(医学版),2011,49(5):29-33. |
| |
| 作者姓名： | 张士杰 陈飞 蒋欣泉 |
| |
| 作者单位： | 1. 山东大学第二医院口腔正畸科,济南,200033
2. 上海交通大学医学院附属第九人民医院口腔颌面外科,上海,200072 |
| |
| 基金项目： | 山东省自然科学基金，山东省科技攻关项目 |
| |
| 摘 要： | 目的研究新型多孔多肽聚乳酸乙醇酸/羟基磷灰石(RGD-PLGA/HA)支架材料的体外细胞相容性。方法采用贴壁法对兔骨髓基质细胞(BMSCs)进行体外矿化诱导培养,扩增后与实验A组(RGD-PLGA/HA)、实验B组(PLGA/HA)分别进行体外复合培养,并通过定性及定量法检测BMSCs在材料表面的黏附能力、增殖活力,验证细胞材料复合体的成骨活性。结果兔BMSCs在每组材料的表面均能生长,经体外诱导后在支架材料的表面形成钙结节,实验A组细胞的黏附及增殖能力均强于实验B组。结论兔BMSCs与新型多孔RGD-PLGA/HA支架材料有良好的相容性。
|
| 关 键 词： | 骨髓基质细胞 多肽聚乳酸乙醇酸/羟基磷灰石支架 组织工程 兔 |
Cell compatibility of a new type polyporous RGD-PLGA/HA scaffold in vitro |
| |
| ZHANG Shi-jie,CHEN Fei,JIANG XIN-quan.Cell compatibility of a new type polyporous RGD-PLGA/HA scaffold in vitro[J].Journal of Shandong University:Health Sciences,2011,49(5):29-33. |
| |
| Authors: | ZHANG Shi-jie CHEN Fei JIANG XIN-quan |
| |
| Institution: | 1.Department of Orthodontics,Shandong University of the second hospital,Jinan 250033,China;2.Department of Oral and Maxillofaeial Surgery,College of Stomatology,Ninth People′s Hospital,Shanghai Jiao Tong University,Shanghai 200072,China) |
| |
| Abstract: | Objective To study the cell biocompatibility of a new type polyporous RGD-PLGA/HA scaffold in vitro.Methods Bone marrow stromal cells(BMSCs) obtained from rabbits were cultured,induced and proliferated in vitro.The cells were incubated with RGD-PLGA/HA in experimental group A and PLGA/HA in group B.The capacity of adhesion and proliferation of BMSCs were qualitatively and quantitatively examined,and the osteogenesis activity of cell composite was tested.Differences between the 2 groups were analyzed.Results... |
| |
| Keywords: | BMSCs RGD-PLGA/HA scaffold Tissue engineering Rabbits |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
