| RNA干扰Bmi-1对胶质瘤细胞增殖的影响 |
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| 引用本文： | 梁君,杨东旭,华磊,王强,潘昕,周秀萍,石琼,于如同. RNA干扰Bmi-1对胶质瘤细胞增殖的影响[J]. 临床神经外科杂志, 2011, 8(5): 252-254. DOI: 10.3969/j.issn.1672-7770.2011.05.009 |
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| 作者姓名： | 梁君 杨东旭 华磊 王强 潘昕 周秀萍 石琼 于如同 |
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| 作者单位： | 徐州医学院附属医院神经外科,221006 |
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| 摘 要： | 目的 初步探讨Bmi-1基因对胶质瘤细胞增殖状况的影响.方法 采用RNA干扰技术沉默U251胶质瘤细胞中Bmi-1基因,RT-PCR检测干扰效果,CCK8观察U251细胞的增殖状况.结果 RNA干扰Bmi-1后,Bmi-1基因表达降低,U251细胞增殖减慢.结论 Bmi-1基因可以促进胶质瘤细胞的增殖,有可能促进了...
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| 关 键 词： | 胶质瘤 Bmi-1 RNA干扰 增殖 |
Effects of RNA interfering Bmi-1 on glioma cells proliferation |
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| Affiliation: | LIANG Jun, YANG Dong-xu, HUA Lei, et al. Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China |
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| Abstract: | Objective To research the effect of B-cell-specific moloney murin leukemia virus insertion site 1 （Bmi-1） Gene on glioma cells proliferation. Methods Bmi-1 gene in U251 glioma cells is silenced by RNA interfering, the expressions of Bmi-1 is detected by RT-PCR. The condition of cells proliferation is observed by CCK8. Results After Bmi-1 in U251 cell was interfered, the expression of Bmi-1 was decreased and the cell proliferation was stepped down. Conclusion The Bmi-1 gene may promote the proliferation of glioma cells, it probably promotes the genesis and development of glioma. |
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| Keywords: | glioma Bmi-1 RNA interfering proliferation |
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