2型糖尿病患者热休克蛋白70基因多态性的变化

目的探讨热休克蛋白70（HSP70）基因多态性与2型糖尿病的关系。方法应用聚合酶链反应-限制性片段长度多态性（PCR．RFLP）方法，检测HSP70基因型在112例2型糖尿病患者和110例对照组的分布情况。结果112例2型糖尿病患者HSP70—1A／A、A／B和B／B3种基因型的分布频率分别为73．2％、23．2％和3．6％，HSP70．2A／A、A／B和B／B3种基因型的分布频率分别是55．4％、34．8％和9．8％，HSP70-hom A／A、A／B和B／B3种基因型频率分别是58．9％、37．5％和3．6％；110例对照组HSP70—1A／A、A／B、B／B基因型分布频率分别是54．5％、40．9％和4．6％，HSP70-2A／A、A／B、B／B3种基因型频率分别是52．7％，36．4％和10．9％，HSP70-hom A／A、A／B、B／B3种基因型频率分别是47．3％、47．3％和5．4％。结论112例2型糖尿病患者HSP70-1A／A、A／B和B／B3种基因型的分布与110例对照组比较差异有统计学意义（P〈0．05），而HSP70-2和HSP70-homA／A、A／B和B／B3种基因型的分布与对照组比较差异无统计学意义（P〉0．05）。

The genetic polymorphism of heat shock protein 70 family in T2DM

Objective To explore the gene polymorphisms of heat shock proteins70 family members（HSP70-1 ,HSPT0-2 ,HSP70-hom） with Type 2 Diabetes Mellitus（T2DM）. Methods The genotypes of 112 patients with T2DM and 110 healthy control subjects were tested with polymerase chain reaction followed by restriction fragment polymorphism analysis for HSP70 gene. Results In T2DM group, HSP 70-1 genotype A/A,A/B and B/B were 73.2% ,23.3% and 3.6% ,HSP70-2 genotype A/A,A/B and B/B were 55.4% ,34.8% and 9.8%, and HSP70-hom genotype A/A,A/B and B/B were 58.9% , 37.5% and 3. 6% respectively; In the control group, HSP70-1 genotype A/A,A/B and B/B were 54.5% ,40.9% ,HSP70-2 genotype A/A,A/B and B/B were 4.6% ,52.7% ,36.4% and HSP70-hom genotype A/A,A/B and B/B were 10.9% ,47.3% ,47.3% and 5.4%, respectively. Conclusion There were significant differences in the distribution of genotypes A/A,A/B and B/B, allele frequencies A,B of HSP70-1 between 112 patients with T2DM and 110 controls （P 〈 0.05 ）. However, there were not significant differences in the distribution of genotypes A/A, A/B and B/B, allele frequencies A, B of HSP70-2 and HSP70-hom between cases and controls（P 〉 0.05）.