星点设计-响应面法优选枳实总黄酮的大孔吸附树脂纯化工艺

目的：优选枳实总黄酮的大孔树脂纯化工艺。方法：选择吸附时间、乙醇体积分数、洗脱用量为自变量，总黄酮吸附-洗脱率为因变量，在单因素试验基础上，采用星点设计-响应面法优选大孔树脂纯化工艺。结果：选择AB-8型大孔树脂，最佳纯化工艺为上样液质量浓度3.5 g·L^-1，pH 6.0，上样量0.6 BV，上样速率1 BV·h^-1，水洗用量1 BV，洗脱速率2 BV·h^-1，吸附时间77 min，乙醇体积分数88%，洗脱剂用量2.4 BV；枳实总黄酮吸附-洗脱率达93.0%，与预测值（92.4%）偏差较小。结论：采用星点设计-响应面法优选的大孔树脂纯化工艺条件稳定可行，预测性好。

Optimization of Purification Process for Total Flavonoids in Aurantii Fructus Immaturus with Macroporous Resin by Central Composite Design-Response Surface Methodology

Objective: To optimize purification process of total flavonoids in Aurantii Fructus Immaturus by macroporous resin. Method: Independent variables were adsorption time, ethanol concentration and eluent volume, while dependent variables was adsorption-elution rate of total flavonoids, central composite design-response surface methodology was adopted to optimize purification technology on the basis of single factor tests. Result: AB-8 macroporous resin was selected, optimum purification process were as follows:added 0.6 BV of sample solution with concentration of 3.5 g·L^-1 and pH 6.0, sample rate 1 BV·h^-1, adsorption time 77 min, water consumption 1 BV, elution rate of 2 BV·h^-1, eluted with 2.4 BV of 88% ethanol.Adsorption-elution rate of total flavonoids was up to 93.0%, which had a small deviation with the predicted value of 92.4%. Conclusion: Optimized purification technology was stable and feasible with high predictability.