山西省家族性乙型肝炎病例中HLA-DRB1基因位点的分析

目的 探讨HLA-DRB1等位基因多态性与家族性乙型肝炎之间的关系.方法 采用聚合酶链反应-特异性序列寡核苷酸（PCR-SSO）探针基因分型技术结合荧光磁珠流式检测仪,对151例家族性乙肝人群的HLA-DRB1基因位点进行了分析.结果 HLA-DRB1＊0701在慢乙肝组的等位基因频率（17.8%）明显高于正常对照组（7.4%）,差异有统计学意义（P＜0.05,A=1/0.367=2.725）;HLA-DRB1＊0401/0403/0405及HLA-DRB1＊1301/1302在正常对照组的等位基因频率（16.2%;4.4%）明显高于慢乙肝组（5.1%;0）,差异有统计学意义（P＜0.05,A=3.602;P＜0.05）.结论 HLA-DRB1＊0701与家族性慢性乙型肝炎易感性相关,可能是家族性慢性乙型肝炎的易感基因或连锁基因;HLA-DRB1＊0401/0403/0405、HLA-DRB1＊1301/1302与家族性慢性乙型肝炎抗性相关,可能是家族性慢性乙型肝炎的抗性基因.宿主的HLA-DRB1可能是预测家族性乙肝转归的主要指标.

Study on HLA-DRB1 alleles in patients with familial hepatitis B in Shanxi

Objective To study the association between HLA-DRB1 alleles and familial hepatitis B. Methods HLA-DRB1 alleles of 151 people in the familial hepatitis B families were detected,by using the the polymerase chain reaction-sequence specific oligonucleotide probing(PCR-SSOP) technique. Results The allele frequency of HLA-DRB1 * 0701 in the chronic hepatitis B(CHB) group was markedly higher than that in the normal control group( 17.8% vs 7.4% ), with significant correlation between them (P ＜0.05, A = 1/0. 367 ≈2. 725 ). The allele frequencies of HLA-DRB1 * 0401/0403/0405 and HLA-DRB1 *1301/1302 in the normal control group( 16. 2% ,4.4% ) were markedly higher than that in the CHB group (5. 1% ,0) ,with statistical significance ( P ＜ 0. 05, A = 3. 602; P ＜ 0. 05 ). The other alleles between the CHB group and the normal control group are no significant differences. Conclusion HLA-DRB1 * 0701 is closely associated with the susceptibility to familial hepatitis B ,and may be the susceptible or linkage gene.HLA-DRB1 * 0401/0403/0405 and HLA-DRB1 * 1301/1302,in inverse proportion to the susceptibility to familial hepatitis B,may be the resistant gene. They may regulate the outcome of familial HBV infection in a patient.