肾素基因新的单核甘酸多态性与原发性高血压

目的扫描肾素基因发现新的多态性，探讨肾素基因新多态性与原发性高血压的关联性。方法运用聚合酶链反应-单链构象多态性（PCR—SSCP）方法，对肾素基因（REN）外显子4-8进行扫描，以发现新的多态性；运用聚合酶链反应．限制性片段长度多态性（PCR-RFLP）确定新的多态性，在日本人212例原发性高血压（EH）者和209例正常血压（NT）者中进行病例对照研究。结果PCR．SSCP法发现外显子4下游第17碱基对处内含子4区域的一个单核苷酸多态性（SNP）T＋17int4G；DNA测序确定多态性。PCR—RFLP确定基因型后结果显示T＋17int4G的基因型频率在原发性高血压和正常血压组中无显著性差异（P〈0．05）。结论内含子4的T＋17int4G SNP与日本人原发性高血压无关联性。

A Novel Nucleotide polymorphism of Renin Gene and Essential Hypertension

Objective To screen coding region of human renin gene to find new polymorphisms and investigate whether there are associations between new polymorphisms of renin gene and essential hypertension(EH).Methods Using the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method, we isolated A single nucleotide polymorphism (SNP) in intron 4 (T 17int4G); We performed an association study using this SNP in 212 hypertensive patients and 209 age-matched normotensive (NT) subjects.Results Distribution of genotype frequency T 17int4G was not significantly different in the two groups.Conclusion T 17int4G SNP was not associated with EH.