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| 腺病毒介导多基因在胆管癌细胞中的表达 | |
| 引用本文： | 王征旭,何振平,吴祖泽,马宽生,刘吉奎,张维维. 腺病毒介导多基因在胆管癌细胞中的表达[J]. 第三军医大学学报, 1999, 21(10): 742-745 |
| 作者姓名： | 王征旭 何振平 吴祖泽 马宽生 刘吉奎 张维维 |
| 作者单位： | 1. 第二军医大学东方肝胆外科医院,上海,200438 2. 第三军医大学附属西南医院肝胆外科中心,重庆,400038 3. 军事医学科学院放射医学研究所百环生物医学研究中心,北京,100000 4. 美国Baxter医疗用品公司基因治疗部 |
| 摘 要： | 研究腺病毒介导的多基因（ＧＭ－ＣＳＦ,Ｂ７－１,ｐ５３、ＩＬ－２）在胆管癌细胞系ＱＢＣ９３９中的表达及对其生长的影响。方法：构建同时含ＧＭ－ＣＳＦ、Ｂ７－１、ｐ５３、ＩＬ－２ ４种目的基因的重组腺病毒载体Ａｄ－ｍｕｌｔｉｇｅｎｅｓ,应用流式细胞仪、ＥＬＩＳＡ、免疫组化等方法。 |
| 关 键 词： | 多基因腺病毒 胆管癌细胞系 基因表达 生长抑制 |
Expression of adenovirus-mediated GM-CSF, B7-1, p53 and IL-2 in cholangiocarcinoma cells line |
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| Wang Zhengxu,He Zhengping,Wu Zuze,Ma Kuansheng,Liu Jikui,Zhang Weiwei. Expression of adenovirus-mediated GM-CSF, B7-1, p53 and IL-2 in cholangiocarcinoma cells line[J]. Acta Academiae Medicinae Militaris Tertiae, 1999, 21(10): 742-745 | |
| Authors: | Wang Zhengxu He Zhengping Wu Zuze Ma Kuansheng Liu Jikui Zhang Weiwei |
| Affiliation: | Wang Zhengxu, He Zhengping, WuZuze, Ma Kuansheng, Liu Jikui, Zhang Weiwei (Department of Hepatobiliary Surgery,Southwest Hospital, Third Military Medical University,Chongqing,400038) |
| Abstract: | Objective: To study the expression rate of adenovirus-mediated human genes of GM-CSF,B7-1, p53 and IL-2 in the cells of cholangiocarcinoma cell line QBC939 and their effects on the growth of the malignant cells. Methods: Recombinant adenovirus was constructed to contain human genes of GM-CSF,B7-1, p53 and IL-2 and the expression rate of the genes in the ce1ls of hurnan cholangiocarcinoma celI line QBC939 was detected with ELISA, immunohistochemistry and FACS. In addition, the growth of the malignant cells was deIineated. ResuIts: Adenovirus-mediated multigenes except IL-2 were expressed in high rate in the malignant cells and they inhibited the growth of the cells. Conclusion: Adenovirus-mediated human genes of GM-CSF, B7-1 and p53 can be expressed in cholangiocarcinoma cell line QBC939 and inhibit the growth of the cells. |
| Keywords: | GM-CSF B7-1 p53 IL-2 cholangiocarcinoma cell line gene expression growth inhibition |
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