首例经自体颗粒细胞线粒体移植的双胞胎儿出生

1名37岁妇女,婚后有2次自然流产史,并发不孕症。来我院2次行体外受精和胚胎移植,由于胚胎质量差,均未妊娠。第3周期对患者的5个成熟卵子进行自体颗粒细胞线粒体移植,4个卵子做胞浆内单精子注射。经线粒体移植后的胚胎质量优于胞浆内单精子注射组。移植3个经线粒体移植后的胚胎,获3胎临床妊娠。妊娠5周时1例胚胎停育,其余2胎发育正常。患者在妊娠前后进行了5次丈夫淋巴细胞免疫治疗。妊娠30周患者因重度妊高征住院治疗,疗效不明显,遂于2003年8月6日妊娠32周时行剖宫产,娩出发育正常的男女2个新生儿。在新生儿病房治疗后健康出院。此为我国大陆地区首例经线粒体移植妊娠出生的胎儿。

First twins born in Mainland China by autologous granular cell mitochondria transfer

A 37-year-old woman with the history of twice spontaneous abortion, who received unfruitful in vitro fertilization and embryo transfer (IVF-ET) for two cycles due to poor embryo quality, underwent the third cycle for autologous granular cell mitochondria transfer of 5 matured oocytes and intracytoplasmic sperm injection (ICSI) in another 4 oocytes. Better em-bryo quality was resulted by the former technique than by the latter, despite that fertilization was achieved in all the 4 oocytes with the latter operation. Four of the 5 oocytes with mitochondria transfer were fertilized and developed into normal embryos, 3 of which were selected for intrauterine transplantation. Clinical pregnancy with triplets was subsequently obtained, but the development of one fetus accidentally stopped at the fifth week of pregnancy. The woman received five times of immunother-apy with her husband's leukocytes before and after the pregnancy. At the 30 th week of pregnancy, the women was admitted for severe pregnancy-induced hypertension syndrome that failed to respond to a two-week treatment course. Cesarean section was subsequently performed and two normal babies, one boy and one girl, were born on August 6th, 2003, who were taken home after care in neonate wards. This is the first report of the birth of babies born by autologous granular cell mitochondria transfer in Mainland China.