三种不同生物学特性细胞在软琼脂中的生长表型分析

背景与目的软琼脂(soft agar)实验一般用来检测细胞的集落形成能力,但很早就有人根据形态学变化用它来检测低等生物的生物学特性。本文探讨利用软琼脂集落形成实验这种方法进行哺乳动物癌细胞表型初步分析的可行性。方法将三种不同生物学特性的细胞(小鼠成纤维细胞NIH3T3,高转移人肺腺癌细胞系Anip973,高浸润人肺腺癌细胞系L18)悬液接种到软琼脂中,分别在1天、5天、14天后观察细胞形态特征。结果第五天开始细胞集落形态出现差异,14天时差异更显著。NIH3T3细胞克隆为椭圆形,中等大小,边界清楚;Anip973克隆周围有大量游走细胞,表现出其转移特性;L18细胞克隆分支多并向周围延伸扩展,与其浸润特性相符。结论软琼脂分析实验是一种良好的根据表型来观察及鉴定哺乳动物细胞生物学特性的方法。

The morphous analyses of three different characteristics cells growing in soft agar

Background and objective Soft agar assay was usually used in colony formation observation of cells,while it was also be empolyed in detecting bionomics of inferior creature.In this article the feasibility of soft agar assay in detecting mammalian cell phenotype is investigated.Methods Three different characteristic cells( L cell NIH3T3;higher metastatic human lung adenoearcinoma cell line Anip973;higher invasive potential human lung adenocarcinoma cell line L18) were seed into soft agar.The morphology of cells was obserned in the first day, the Fifth day and the fourteenth day,respectively.Results In the fifth day,morphology of the three kinds cells ap- peared difference and in the fourteenth day the difference became more notable.NIH3T3 cell clones were ellipse and moderate size,their boundary were distinct;Anip973 cell clones were surrounded with plenty of migratory cells, which was consistent with higher metastatic characteristics of the cells.L18 cell clones showed lots of branchs and extended toward environment.Conclusion Soft agar assay is a good method in evaluating and observed biological property of mammalian cells according to their phenotypes.