Excellent reproducibility of laser speckle contrast imaging to assess skin microvascular reactivity |
| |
| Affiliation: | 1. Univ. Grenoble Alpes, HP2, 38000, France;2. Clinical Pharmacology Unit, Inserm CIC003, Grenoble University Hospital, 38043, France;3. Vascular Medicine Department, Grenoble University Hospital, 38043, France;1. Centre of Research Excellence to Reduce Inequality in Heart Disease, Mary MacKillop Institute for Health Research, Australian Catholic University, Melbourne, Australia;2. School of Exercise Science, Australian Catholic University, Melbourne, Australia;3. Avignon University, LAPEC EA4278, F-84000 Avignon, France;4. Clinical Pharmacology, Grenoble University Hospital; and Inserm U1042, HP2, Université Grenoble Alpes, F-38000 Grenoble, France;5. Department of Endocrinology and Diabetes, St Vincent''s Hospital, Melbourne, Australia;6. The University of Melbourne, Melbourne, Australia;7. Mary MacKillop Institute for Health Research, Australian Catholic University, Melbourne, Australia;1. Research Laboratory and Academic Division of Clinical Rheumatology, Department of Internal Medicine, IRCCS A.O.U. San Martino-IST, University of Genova, Genoa, Italy;2. Department of Rheumatology, Ghent University Hospital, Ghent University, Ghent, Belgium;1. Laboratory of physiology, vascular investigations, University Hospital, 49933 Angers Cedex 09, France;2. LISA-Laboratoire d’Ingénierie des Systèmes Automatisés, LUNAM Université, Université d’Angers, Angers, France;3. CNRS UMR 6214, INSERM U1083, Université d''Angers, UFR de Médecine, rue Haute de Reculée, 49045 Angers, France;4. Depart of dermatology, University Hospital, 49933, Angers cedex 09, France |
| |
| Abstract: | ObjectiveWe compared the inter-day reproducibility of post-occlusive reactive hyperemia (PORH) assessed by single-point laser Doppler flowmetry (LDF) and laser speckle contrast analysis (LSCI), and the reproducibility of local thermal hyperemia (LTH) assessed by LDF, laser Doppler imaging (LDI) and LSCI. We also tested whether skin blood flow assessment by LDF and by LSCI are correlated.MethodsSkin blood flow was evaluated during PORH and LTH using LDF, LDI (for LTH only) and LSCI on the forearms of healthy volunteers, at a 7 day interval. Data are expressed as cutaneous vascular conductance (CVC), as a function of baseline and scaled to the thermal plateau. Reproducibility is expressed as within subject coefficients of variation (CV, in %) and intra-class correlation coefficients (ICC).ResultsTwenty-eight healthy participants were enrolled in this study. The reproducibility of the PORH peak CVC was better when assessed with LSCI compared to LDF (CV = 8%; ICC = 0.76 and CV = 30%; ICC = 0.54, respectively). Inter-day reproducibility of the LTH plateau was better when assessed with LSCI or LDI than LDF (CV = 15%, ICC = 0.66; CV = 17%, ICC = 0.51 and CV = 42%, ICC = 0.28 respectively). Finally, we observed significant correlation between simultaneous LDF and LSCI measurements of the PORH peak CVC (R = 0.54; p = 0.001).ConclusionThe recently developed LSCI technique showed very good inter-day reproducibility for assessing PORH and LTH. Moreover, we showed significant correlation between LSCI and single-point LDF for PORH. However, more data are needed to evaluate the linearity between the LSCI signal and skin blood flow. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
