Influenza A virus replication is inhibited in IFN-λ2 and IFN-λ3 transfected or stimulated cells |
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| Institution: | 1. Institute of Virology, Slovak Academy of Sciences, Dubravska cesta 9, 845 05 Bratislava, Slovak Republic;2. Centre for Molecular Medicine, Vlarska 3-7, 831 01 Bratislava, Slovak Republic;3. Comenius University, Department of Microbiology and Virology, Bratislava, Slovak Republic;1. Division of Pediatric Allergy/Immunology, Johns Hopkins School of Medicine, Baltimore, Md;2. Division of Pediatric Allergy/Immunology, Boston Children''s Hospital, Harvard Medical School, Boston, Mass;3. Department of Environmental Health Sciences, Columbia University, New York, NY;4. Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Md;5. Department of Public Health, Dell Medical School, University of Texas, Austin, TX;1. Division of Basic and Clinical Immunology, Department of Medicine, University of California–Irvine, Irvine, California, USA;2. Cellular and Molecular Immunology Laboratory, Gavin Herbert Eye Institute, University of California–Irvine, Irvine, California, USA;1. The Kirby Institute, UNSW Australia, Sydney, NSW, Australia;2. Cluster Infectious Diseases, GGD Public Health Service of Amsterdam, Amsterdam, The Netherlands;3. Department of Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, CA, USA;4. Harvard Medical School, Boston, MA, USA;5. Tufts Medical School, Boston, MA, USA;6. Abbvie, Chicago, IL, USA;7. Department of Medicine, Johns Hopkins Medical Institutions, Baltimore, MD, USA;8. Burnet Institute, Melbourne, VIC, Australia;9. Department of Epidemiology and Preventive Medicine, Monash University, Melbourne, VIC, Australia;10. CRCHUM, Université de Montréal, Montreal, QC, Canada;11. Academic Medical Center, Amsterdam, The Netherlands;12. Inflammation and Infection Research Centre, School of Medical Sciences, UNSW Australia, Sydney, NSW, Australia;1. Department of Allergology and Internal Medicine in children, Copernicus Hospital, al. Pilsudskiego 71, 90-329, Lodz, Poland;2. Department of Social and Preventive Medicine, Medical University of Lodz, Lodz, Poland |
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| Abstract: | Interferons lambda (IFN-λ) are the most recently defined members of the class III cytokine family. To investigate whether IFN-λ2 and IFN-λ3 displayed antiviral activity against influenza A virus (IAV), a number of cell lines induced with IFNs – as well as two established cell lines (A549-IFN-λ2 and A549-IFN-λ3) – were infected with IAV. Our results indicate that IFN-λ2 has statistically significant antiviral activity in A549-IFN-λ2 (P = 0.0028) although less so than IFN-λ3, which reduced viral titer to 10% (P < 0.0001). The reverse was observed for cells treated with IFNs, with IFN-λ2-treated A549 cells inhibiting IAV infection more efficiently than IFN-λ3-treated A549 cells. The antiviral effect on IFN-stimulated cells was most apparent on Vero cells (compared with MDCK and HeLa). Both IFNs significantly inhibited IAV replication and inhibition was observed in a dose-dependent manner, with an optimal IFN concentration of 20 ng/ml. IFN-λ2 was more potent than IFN-λ3 on Vero cells while IFN-λ3 appeared more efficient than IFN-λ2 on MDCK and HeLa cells. |
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