IL-13抑制人肾小球系膜细胞IL-12的表达

为了探讨白细胞介素 13(IL 13)对体外培养的人肾小球系膜细胞产生白细胞介素 12 (IL 12 )的影响。我们用脂多糖(LPS 10 μg/ml) ,不同浓度的IL 13对系膜细胞培养 ,分别采用ELISA法和半定量RT PCR法检测细胞上清液的IL 12和系膜细胞IL 12p4 0mRNA表达。结果提示 5 %NCSRPMI 16 4 0基础培养条件下的系膜细胞未检测到IL 12蛋白分泌及其mRNA表达。在LPS刺激下系膜细胞的IL 12p4 0mRNA表达加强 ,并分泌出大量的IL 12。IL 13在 1～ 10 0ng/ml浓度范围内对LPS诱导的系膜细胞IL 12分泌及IL 12p4 0mRNA表达的抑制作用呈剂量依赖趋势。本研究认为IL 13可能通过抑制IL 12的产生 ,而调整了体内Th1/Th2细胞因子平衡 ,作为抗炎性细胞因子在肾小球肾炎发病机制中发挥一定作用

Inhibitory Effect of IL-13 on IL-12 Production in Mesangial Cells

To investigate the effect of interleukin-13 (IL-13) on interleukin-12 (IL-12) production in mesangial cells,mesangial cells were cultivated with LPS (10 μg/ml) or/and various concentration of IL-13 and the protein synthesis of IL-12 in mesangial cells was measured by ELISA. The expression of IL-12 mRNA in mesangial cells was evaluated by RT-PCR. The results showed that both mRNA and protein of IL-12 were not produced in mesangial cells when treated by 5% NCS RPMI 1640. However,the production of IL-12 in mesangial cells stimulated with LPS was significantly increased. IL-13 (1～10 ng/ml) inhibited the protein and mRNA expression of IL-12 in a dose-dependent manner. It concludes that IL-13 inhibits IL-12 expression induced by LPS in mesangial cells. IL-13 may regulate renal injury and immune responses by balancing Th1/Th2 in glomerulonephritis.