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Beclin 1 expression is an independent prognostic factor for gastric carcinomas |
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| Authors: | Miao Yu Wen-feng Gou Shuang Zhao Li-jun Xiao Xiao-yun Mao Ya-nan Xing Hiroyuki Takahashi Yasuo Takano Hua-chuan Zheng |
| Affiliation: | 1. Department of Surgical Oncology, The First Affiliated Hospital of China Medical University, Shenyang, 110001, China 2. Department of Biochemistry and Molecular Biology, Institute of Pathology and Pathophysiology, College of Basic Medicine, China Medical University, Shenyang, 110001, China 3. Department of Immunology, School of Basic Medicine, Chengde Medical Collge, Chengde, 067000, China 4. Clinical Research Institute, Kanagawa Cancer Center, Yokohama, 241-0815, Japan |
| Abstract: | Beclin 1, an important autophagy-related protein in human cells, is involved in autophagy, differentiation, anti-apoptosis, and cancer progression, which is increased during periods of cell stress and extinguished during the cell cycle. In order to clarify the role of Beclin 1 in gastric carcinogenesis and subsequent progression, its expression was examined by immunohistochemistry and in situ hybridization (ISH) on tissue microarrays containing gastric carcinomas, adjacent non-neoplastic mucosa, and metastatic lymph node. Gastric carcinoma tissue and cell lines were studied for Beclin 1 expression by Western blot or RT-PCR, respectively. The results demonstrated that Beclin 1 was distinctively expressed in GES-1, AGS, BGC-823, GT-3 TKB, HGC-27, KATO-III, MGC-803, MKN28, MKN45, SCH, SGC-7901, or STKM-2 at both mRNA and protein levels. However, Beclin 1 mRNA was highly expressed in gastric carcinoma than matched mucosa by real-time PCR and ISH (P?P?P?P?P?P?P?P? |
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