罐组逆流提取工艺参数的多指标优化方法研究

 目的利用多指标综合评价和回归分析,建立罐组逆流提取工艺参数的多指标综合优化方法。方法以三七皂苷R₁、人参皂苷Rg₁、总皂苷和浸出物含量为优化目标,采用正交试验设计,考察乙醇浓度、溶剂用量、阶段提取时间和提取温度等因素对优化目标的影响;利用回归分析方法建立了各优化目标与考察因素间的数学模型,结合方差分析得到三七罐组逆流提取最佳工艺参数,并将罐组逆流提取工艺与其他提取方式进行比较。结果通过多指标综合优化方法得到三七罐组逆流提取的最佳工艺条件为体积分数70%乙醇、8倍药材量溶剂、阶段提取时间30 min、提取温度50℃,与其他提取工艺相比,在保证较高收率的前提下,温度可以降低50%左右,溶剂节省50%～70%,单位时间的处理量增大。结论建立了罐组逆流提取工艺参数的多指标综合优化方法,并成功运用于三七中皂苷类活性成分的提取,得到三七罐组逆流提取最佳工艺条件。

Study on Process Parameters in Multi-Stage Countercurrent Extraction by Multi-Index Optimization Method

OBJECTIVE To establish the optimization method of process parameters in multi-stage countercurrent extraction by the multi-index optimization and regression omalysis.METHODS The extraction yields of notoginsenoside R₁,ginsenoside Rg₁,panax notoginseng saponins(PNS) and extractives were considered as the optimization index.Orthogonal design method was used to optimize the process parameters,including contents of alcohol,solvent volume,extraction time and temperature.The models were established according to the index and the parameters by regression analysis,and the optimal process parameters were obtained by variance analysis.Also the multi-stage countercurrent extraction was compared with other extraction methods.RESULTS The optimal process conditions of multi-stage countercurrent extraction of notoginseng consisted of 70% alcohol,the solvent/sample ratio of 8 mL·g^-1,30 min and 50 ℃.Compared with the other processes,the multi-stage countercurrent extraction was processed at lower temperature,with 50%～70% less solvent consumption,and more herb was extracted in unit time. CONCLUSION The multi-index optimization method of multi-stage countercurrent extraction is established and successfully applied in the extraction of saponins from notoginseng.