| Abstract: | We showed mesenchymal progenitor cells(MPC) contain various cells, and the differentiational capacities and effects on wound healing were different each other. Bone marrow of the femur of adult F344 rat was suspended into culture medium and plated on a plastic dish.10–15 passaged cells were cloned in 100 types cells. For investigation of the differentiational capacity, the cells were treated by differentiational medium. The capacity of myogenesis was examined immunohistochemically with anti‐skeletal muscle myosin antibody. Osteochondrogenesis were investigated by alcian blue staining and adipogenesis by oil red O staining. The 53% of cells showed myogenesis, 78% osteochondrogenesis and 100% adipogenesis. The one type of cell showed amazing adipogenesis pattern, which we named “O” cell. Next we injected MPC and this “O” cell into the rats’ dorsal skin, and 1 cm full thickness incisional wounds were made immediately after. 2 weeks later, wounds were harvested and examined histologically. The wounds transplanted with high dose of MPC healed with very fine scar and collagen fibers were thick and aligned like normal dermis. But by transplanting the “O” cells, wounds healed with ordinary scar formation. By tansplantating mesenchymal progenitor cells, lesion healed with less scars. And there was a possibility that the MPC responded to the wound healing and regenerated dermal structure nearly normally. But the wounds healed not well by transplanting just “O” cell although it possesses the amazing differentiational capacity. The data demonstrate that plural types cells were necessary for wound healing. |
