茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡 |
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引用本文: | 石林,秦虹,刘适,崔云甫. 茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡[J]. 中华实验外科杂志, 2009, 26(1): 842-844. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.009 |
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作者姓名: | 石林 秦虹 刘适 崔云甫 |
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作者单位: | 哈尔滨医科大学附属第二医院普外一科,150001;哈尔滨医科大学公卫学院; |
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摘 要: | Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.
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关 键 词: | 胰腺癌 没石子儿茶素没石子酸酯 脱噬作用 |
Regulation of akt pathway in apoptosis of pancreatic cancer cells induced by polyphenols |
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Abstract: | |
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Keywords: | Pancreatic carcinomaEGCGApotosis |
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