骨肉瘤患者p53基因的改变及其与预后的关系

目的：研究人骨肉瘤中ｐ５３蛋白表达及Ｐ５３基因突变及其与预后的关系。方法：免疫组织化学染色观察人骨肉瘤中Ｐ５３蛋白表达，银染ＰＣＲ－ＳＳＣ民埋从瘤中Ｐ５３基因５－８外显子点突变状态。预后分析用Ｋａｐｌａｎ－Ｍｅｉｅｒ生存曲线及Ｌｏｇｒａｎｋ时序检验。结果：人骨肉瘤中Ｐ５３蛋白阳性率为２９．１７％，ＳＳＣＰ检测Ｐ５３基因５－８外显子占突变率为２３．９６％，Ｐ５３蛋主ＳＳＣ生患者与其阴性患者间总和生存

Expression and mutation of p53 gene in human osteosarcoma and their relationship to prognosis

Purpose To investigate the expression and mutation of p53 gene in human osteosarcoma andtheir relationship to prognosis. Methods The expression of p53 protein was detected by immunohistochemicalstain. The point mutation of p53 gene was detected by silver staining PCR-SSCP in paraffin-embedded osteosar-coma. The prognosis was analyzed by Kaplan-Meter survival curve and Logrank test. Results The positive rateof p53 protein expression and point mutation were 29. 17% and 23. 96 % individully. The positive coincidencerate between p53 gene mutation and protein expression was 82. 14 %, mutation in exon 7 was highest among the5, 6, 7, 8 exon. p53 protein positive and SSCP positive patients had a worse out come in the first year than theirnegative conterparts, but there is no difference overall(P >0. 05 ). Conclusion The protein expression and mu-tation of p53 gene of osteosarcoma might be a predict variable in judgement of malignancy, metastasis and prog-nosis. And, in some extent, the p53 protein expression can reflect the p53 gene mutation aproximately.