雄激素对下丘脑室旁核中注射L-精氨酸诱发阴茎勃起的影响

目的 :探讨雄激素对下丘脑室旁核 (PVN)中注射L 精氨酸诱导的阴茎勃起是否有影响。　方法 :健康成年雄兔随机分成去势组、去势后雄激素替代组及假手术对照组 3组 (每组 8只 )。饲养 1个月后 ,监测在PVN中注入L 精氨酸后海绵体窦内压 (ICP)的变化及测定每只雄兔PVN组织中一氧化氮合酶 (NOS)活性。　结果 :去势组中ICP最高值均值为 (2 2 .6 4 0± 4 .76 7)mmHg(1mmHg =0 .133kPa) ,NOS活性均值为 (0 .80 7±0 .188)U/mg·prot,与替代组或假手术对照组相比 ,差异均有显著性 (P <0 .0 1)。替代组ICP最高值均值及NOS活性分别为(38.14 6± 4 .90 7)mmHg和 (1.4 5 7± 0 .2 93)U/mg·prot,与假手术对照组相比 ,差异无显著性 (P >0 .0 5 ) ,假手术对照组ICP最高值均值及NOS活性分别为 (39.991± 3.0 6 8)mmHg和 (1.5 2 8± 0 .2 4 0 )U/mg·prot。 　结论 :一氧化氮是PVN中诱导阴茎勃起的神经递质之一 ,且L 精氨酸在PVN中诱导的阴茎勃起需要雄激素的维持

Effect of Androgen on Penile Erection Induced by L-arginine in Paraventricular Nucleus

OBJECTIVES: To investigate the relationship between penile erection induced by L-arginine in paraventricular nucleus (PVN) and androgen. METHODS: Twenty-four mature male rabbits were randomly divided into three groups which were castrate, castrate with testosterone propionate (T) replacement and pseudo-operation. One month later, the highest intracavernous pressure (ICP) and nitric oxide synthase (NOS) of each PVN tissue were measured after infusing L-arginine into the PVN. RESULTS: The mean ICPs of the highest values in castrate, castrate given T replacement and pseudo-operation groups were (22.640 +/- 4.747) mmHg, (38.146 +/- 4.907) mmHg and (39.991 +/- 3.068) mmHg, respectively. The mean value of NOS activity of each group were (0.807 +/- 0.188) U/mg.prot, (1.457 +/- 0.293) U/mg.prot and (1.528 +/- 0.204) U/mg.prot, respectively. The ICP and NOS activity in castrate were different statistically from T replacement and pseudo-operation group (both P < 0.01). T replacement group had no statistical difference from pseudo-operation group(P > 0.05). CONCLUSIONS: Nitric oxide (NO) is one of the neurotransmitters in PVN particularly relevant for the control of penile erection. Penile erection induced by L-arginine in PVN depends on androgen.