全国中药资源普查丹参药材农药残留检测

目的:了解全国范围内丹参农药残留现状,并为制定丹参农残含量标准提供数据基础。方法:建立检测144种农药残留的气相色谱-串联质谱法和85种农药残留的液相色谱-串联质谱法,测定全国第四次中药资源普查所收集的来源于安徽、山西、河南、河北及四川省的40批丹参样品中农药残留量,并根据2015年版《中国药典》一部丹参项下含量测定的规定对其丹参酮类和丹酚酸B成分进行含量测定,应用SAS 8.2软件分析研究丹参农药残留量与丹参酮类、丹酚酸B成分含量的相关性。结果:40批丹参样品中有20批检测出有农药残留,均为非禁用或限用农药。其中19批检出草克净/嗪草酮,3批检出氟乐灵,8批丹参样品丹参酮类和丹酚酸B成分含量不符合2015年版《中国药典》规定;草克净/嗪草酮的残留量与丹参酮类、丹酚酸B均没有相关性。结论:丹参药材栽培过程中存在非常普遍的农药使用情况,使用地区广泛,农药的种类趋于一致。草克净/嗪草酮的使用对丹参中丹参酮类、丹酚酸B的生成和代谢没有影响。该研究为全国范围内丹参农药残留现状及为丹参农残含量标准制定提供了数据支撑和科学依据。

Detection Pesticide Residues of Salviae Miltiorrhizae Radix et Rhizoma in National Survey on Chinese Material Medica Resources

Objective:To study the current situations of pesticide residues of Salviae Miltiorrhizae Radix et Rhizoma in China,in order to further provide data base for formulating the pesticide residues standard of Salviae Miltiorrhizae Radix et Rhizoma. Method:A gas chromatography tandem mass spectrometriy(GC-MS/MS) for detecting 144 kinds of pesticide residues and a ultra-high liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for detecting 85 kinds of pesticide residues were established,in order to determine the pesticide residues of the 40 batches of samples collected from Anhui,Shanxi,Henan,Hebei and Sichuan provinces during the fourth national survey on traditional Chinese medicine resources on the basis of the China Pharmacopoeia (2015 edition). SAS 8.2 software was applied in a correlation analysis. Result:Pesticide residues were detected in 20 batches,all of the pesticides were not banned or restricted pesticides. Metribuzin was detected in 19 batches,and trifluralin was detected in 3 batches. A total of 8 batches of samples of content did not reach the requirements of China Pharmacopoeia (2015 edition). Metribuzin,tanshinones and salvianolic acid B had not the correction. Conclusion:In the process of the cultivation of Salviae Miltiorrhizae Radix et Rhizoma, pesticides is commonly used. The use of oxalazine/azine has no impact on the production and metabolism of danshenone and danfolic acid B. This study on the current situations of pesticide residues of Salviae Miltiorrhizae Radix et Rhizoma in China provides data support and scientific basis for formulating the pesticide residues standard of Salviae Miltiorrhizae Radix et Rhizoma.